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Biological sample imaging equipment

A technology of biological samples and imaging equipment, which is applied in the field of biological instruments, can solve the problems of inability to conveniently control plant growth conditions and meet the needs of scientific research scenarios, and achieve the effect of reducing interference

Active Publication Date: 2021-11-19
MICROLENS TECH CO LTD BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The inventor found that this method cannot conveniently control the growth conditions of plants, and cannot meet the needs of scientific research scenarios

Method used

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  • Biological sample imaging equipment
  • Biological sample imaging equipment
  • Biological sample imaging equipment

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0340] Example 1: Studying cabbage seed germination and seedling growth using a large horizontal petri dish stand 1. Sterilization of the petri dish: disassemble the parts of the petri dish, wash with detergent and water, rinse three times with distilled water, and dry at room temperature; Install the sealing strip in the groove of the tank and fix the front cover with screws, and seal it with the top cover into a paper sealing bag. After sterilized by ethylene oxide gas, disassemble the paper bag in the ultra-clean workbench and take it out for use;

[0341] 2. Sterilize the surface of cabbage seeds: put an appropriate amount of seeds in a 1.5mL centrifuge tube, soak in 75% alcohol + 0.01% Triton X-100 and shake fully for 15 minutes, pour off the liquid; add 95% alcohol to rinse once, pour off the liquid ;Tighten the tube cap after air-drying thoroughly in the ultra-clean workbench;

[0342]3. Prepare medium: 4.33g / L Murashige-Skoog salt, 10g / L sucrose, 3g / L Phytagel plant ge...

example 2

[0348] Example 2: An example of microscopic phenotyping to study the dynamic details and mechanism of shade avoidance responses of Arabidopsis seedlings

[0349] 1. Imaging module installation: install the microscopic phenotype detection module on the dovetail guide rail 76 of the three-dimensional displacement control module, and lock it through the positioning hole 77; the lens of the microscopic phenotype detection module uses a 4-fold magnified bi-telecentric lens;

[0350] 2. Installation of the plant cultivation module: install 16 standard small vertical culture dish racks 36 on the frame fixing parts 35 of the eight-way main bracket; insert the wiring of the top cultivation light source 44 into the electrical socket interface 34, and the top cultivation light source Use a three-in-one LED lamp bead with three monochromatic lights: 660nm red light, 735nm far-red light, and 450nm blue light;

[0351] 3. Seed sterilization: the same as the seed sterilization method introdu...

example 3

[0362] Example 3: An example of microscopic phenotyping to study the rapid response of Arabidopsis seedlings to ethylene growth inhibition

[0363] 1. Imaging module installation, seed sterilization and medium configuration are the same as Example 2;

[0364] 2. Plant sample preparation: Use tweezers to sow sterilized seeds on the surface of the culture medium in an ultra-clean workbench, and arrange them in a row with an interval of about 5mm; multiple rows can be sown in each petri dish, with an interval of 20mm between each row; cover the petri dish , use a parafilm to seal; place it in the dark at 4°C after imbibition for 4 days, take it out and wrap it in aluminum foil, place it vertically at 22°C in the dark and culture it for 2 days, the Arabidopsis seedlings should be close to the surface of the medium and vertical Growth; keep only dim green LED bulbs for illumination in a completely dark room, open the petri dish in the ultra-clean workbench, select plants in good gr...

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Abstract

The embodiment of the present disclosure discloses a biological sample imaging device, which includes an incubator, an observation window is set on one side of the incubator; a biological sample cultivation module is set inside the incubator; an imaging module is set in the incubator Outside the incubator, images of the biological samples cultivated in the biological sample cultivation module are collected through the observation window, so that the environment in the incubator can be conveniently controlled while dynamically photographing the growth process, adapting to various scientific research scenarios. Requirements, in addition, reduce the interference of the imaging module with biological samples due to the isolation of the inside and outside of the incubator.

Description

technical field [0001] The present disclosure relates to the technical field of biological instruments, in particular to a biological sample imaging device. Background technique [0002] In traditional botanical scientific research, limited by experimental means, only the static state of plant phenotype (characteristics or parameters describing plant growth state) at a certain time section can be studied, which reflects all biological conditions before that time point. Cumulative effects of dynamic processes. However, the growth and development of plants is a combination of a series of time-space specific processes, and only by observing the dynamic process can we more fully understand the time-space specificity of plant growth and development regulation. [0003] In the basic scientific research of botany, including but not limited to plant resistance to environmental stress (anti-high temperature, anti-freeze, anti-salt, anti-hypoxia, anti-oxidative damage), light signal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N21/76G01N21/64A01G9/16A01G9/24
CPCG01N21/84G01N21/76G01N21/64A01G9/16A01G9/246G01N2021/8466G01N2021/8411Y02A40/25C12M41/14C12M41/34C12M27/00C12M41/46G01N21/6456A01G31/02A01G9/26Y02P60/21G01N21/6452G01N21/6486
Inventor 纪玉锶
Owner MICROLENS TECH CO LTD BEIJING
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