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Application of TOX3 gene overexpression as liver cancer prognosis marker

A technology for gene overexpression and liver cancer prognosis, applied in the field of molecular biology, can solve the problems of limited research on CD155 regulation

Active Publication Date: 2021-03-19
PEOPLES HOSPITAL PEKING UNIV
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  • Abstract
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Problems solved by technology

Despite the critical role of cellularly expressed CD155 within the tumor microenvironment of HCC, research on CD155 regulation is still limited

Method used

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  • Application of TOX3 gene overexpression as liver cancer prognosis marker
  • Application of TOX3 gene overexpression as liver cancer prognosis marker
  • Application of TOX3 gene overexpression as liver cancer prognosis marker

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Embodiment 1

[0031] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular Cloning Experiment Manual (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions. Example 1 Application of genome-wide CRISPR activation library screening found that TOX3 can inhibit the expression of checkpoint inhibitor CD155

[0032] Although antibody drugs related to immune checkpoints have gradually begun to be used clinically, the regulatory mechanisms of these immune checkpoints are still less studied. The present invention establishes a set of key gene technology for screening and regulating immune checkpoints based on the CRISPR library. Through this technology, we have screened the key regula...

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Abstract

The invention discloses application of TOX3 gene overexpression as a liver cancer prognosis marker. According to the application, a whole genome CRISPR activation library is utilized for screening, and the result shows that TOX3 can inhibit expression of a checkpoint inhibitor CD155. TCGA liver cancer RNA-seq data is further utilized for conducting immune infiltration analysis, the result shows that the expression level of TOX3 is in significant positive correlation with the infiltration frequency of NK cells in liver cancer tissue, in combination with the inhibition effect of CD155 on the NKcells, that TOX3 can activate the NK cells by inhibiting CD155 is further prompted, and therefore prognosis of a liver cancer patient is promoted. Therefore, TOX3 has certain potential application value as a prognosis index in liver cancer.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to the application of TOX3 gene overexpression as a liver cancer prognosis marker. Background technique [0002] Primary liver cancer (HCC) is one of the most common and aggressive malignant tumors in clinical practice. There are about 500,000 new cases every year in the world, and the trend is increasing year by year. Liver cancer has the characteristics of multiple blood vessels, high degree of malignancy, fast growth, wide range of metastasis and high recurrence rate, and the clinical treatment effect is not ideal. The most effective treatment for primary liver cancer is surgical resection, but the postoperative recurrence rate is high, with a tumor recurrence rate of 40% within 5 years, and most patients have reached the advanced stage of the disease when they see a doctor, because they do not meet the indications for surgical treatment and The chance of radical treatment is lo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 陈红松房琼璇陈冬波邓康健廖维甲谢兴旺陈谱
Owner PEOPLES HOSPITAL PEKING UNIV
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