Application of overexpression of tox3 gene as a prognostic marker in liver cancer

A technology for gene overexpression and prognosis, applied in the field of molecular biology, can solve the problems of limited research on CD155 regulation

Active Publication Date: 2021-09-24
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

Despite the critical role of cellularly expressed CD155 within the tumor microenvironment of HCC, research on CD155 regulation is still limited

Method used

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  • Application of overexpression of tox3 gene as a prognostic marker in liver cancer
  • Application of overexpression of tox3 gene as a prognostic marker in liver cancer
  • Application of overexpression of tox3 gene as a prognostic marker in liver cancer

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Embodiment 1

[0031] The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention. If it is not particularly indicated, the embodiments are subject to the conventional experimental conditions, such as Sambrook et al. (Sambrook J & Russell DW, Molecular Cloning: A Laboratory Manual, 2001), or in accordance with the conditions recommended by the manufacturer's manual. Example 1 Application All Genome CRISPR Activation Library Screen Discovery Tox3 can suppress expression of checkpoint inhibitor CD155

[0032] Although antibody drugs related to immunological examination have gradually been applied clinically, there is still less research on the regulatory mechanisms of these immune checkpoints. The present invention establishes a key genetic technology for screening regulatory immunological examination points based on the CRISPR library. Through this technology, we screened the key regulatory gene Tox3 of CD155, which will provide theore...

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Abstract

The invention discloses the application of TOX3 gene overexpression as a liver cancer prognosis marker. The present invention uses genome-wide CRISPR activation library screening to find that TOX3 can inhibit the expression of checkpoint inhibitor CD155. Further analysis of immune infiltration using TCGA liver cancer RNA-seq data found that the expression level of TOX3 was significantly positively correlated with the infiltration frequency of NK cells in liver cancer tissues. Combined with the inhibitory effect of CD155 on NK cells, it was further suggested that TOX3 may activate NK cells by inhibiting CD155 , which is beneficial to the prognosis of liver cancer patients. Therefore, TOX3 has a certain potential application value as a prognostic indicator in liver cancer.

Description

Technical field [0001] The present invention relates to the field of molecular biology, in particular, the application relates to TOX3 overexpression in the liver as prognostic markers. Background technique [0002] Hepatocellular carcinoma (HCC) is the most common clinical, one of the most aggressive malignant tumors worldwide about 500,000 new patients every year, and increasing year by year. Liver blood vessels having multiple, highly malignant, fast growth rate, a wide range of transfer and high rate of recurrence, clinical treatment is not ideal. Treatment is most effective treatment for primary liver cancer is surgical resection, but a higher recurrence rate, tumor recurrence rate was 40% in five years, but at a late stage when the disease most patients treatment, because they do not meet the indications for surgical treatment and there is a high recurrence rate after the loss of the opportunity to question radical treatment, therefore, to seek new prognostic markers and po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 陈红松房琼璇陈冬波邓康健廖维甲谢兴旺陈谱
Owner PEOPLES HOSPITAL PEKING UNIV
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