Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of preparation method of multi-layer coated probiotic microcapsule
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microcapsules and probiotics, applied in the field of microorganisms, can solve the problems of porous surface, low survival rate of probiotics, poor stability, etc., and achieve the effect of improving stress resistance
Active Publication Date: 2022-02-18
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
View PDF10 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
In order to solve the problem of low survival rate and instability of probiotics in use, the use of microcapsule embedding technology to protect probiotics has become a hot research topic at home and abroad.
However, most of the existing microcapsule coating technologies and products have the problem of surface porosity, especially the single-layer microcapsules have a pore size of 500-900 nanometers, and the stability is not good. After entering the human or animal digestive tract, most It is difficult to withstand the effects of gastric acid, bile acid and pancreatic juice with low pH value; when applied to livestock and poultry feed, it is difficult to withstand the high temperature and high pressure impact of feed pelleting; unable to meet requirements
In addition, some technical processes are not suitable for large-scale production, which seriously affects the activity of the coated probiotics and the industrialization and application of their products
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0026] Embodiment 1: the preparation of multi-layer coated Enterococcus faecium microcapsule product
[0027] (1) preparing concentration is the sodium alginate solution of 10g / L in the feeding tank of fermentation system;
[0028] (2) Weigh calcium carbonate at a ratio of 1:1 to the sodium alginate mass ratio, add it to the feeding tank, mix evenly, sterilize, then cool to 37°C, add Enterococcus faecium at the end of logarithmic growth Seed liquid, to a probiotic density of 1 x 10 6 -5×10 6 cfu / ml, fully mixed as the water phase;
[0029] (3) Add Span 80 as the oil phase to the liquid paraffin, the volume percentage of the Span 80 is 0.1%, and add the oil phase to the fermenter for sterilization;
[0030] (4) prepare the calcium chloride solution of 0.1mol / L in another feeding tank as a settling agent, and sterilize;
[0031] (5) At a stirring speed of 400rpm, according to the ratio of the volume ratio of the water phase to the oil phase of 1:3, slowly transfer the water ...
Embodiment 2
[0036] Embodiment 2: the preparation method of multi-layer coated Lactobacillus plantarum microcapsule product
[0037] (1) preparation concentration is the sodium alginate solution of 20g / L in the feeding tank of fermentation system;
[0038] (2) Weigh calcium carbonate in a ratio of 1:5 to the sodium alginate mass ratio, add it to the feeding tank, mix evenly, sterilize, then cool to 37°C, add Lactobacillus plantarum at the end of logarithmic growth Seed liquid, to a probiotic density of 1 x 10 6 -5×10 6 cfu / ml, fully mixed as the water phase;
[0039] (3) Add Span 80 as the oil phase to the liquid paraffin, the volume percentage of the Span 80 is 0.3%, and the oil phase is added to the fermenter, and sterilized;
[0040] (4) prepare the calcium chloride solution of 0.3mol / L in another feeding tank as a settling agent, and sterilize;
[0041] (5) At a stirring speed of 500rpm, according to the ratio of the volume ratio of the water phase to the oil phase of 1:5, slowly tra...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
particle size (mesh)
aaaaa
aaaaa
molecular weight
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a preparation method of multi-layer coated probiotic microcapsules. The preparation method includes coating the probiotics before microencapsulation and coating after fermentation, so as to prepare multilayer composite coated probiotic microcapsules. The preparation method of the invention plays a great role in maintaining the survival and stability of the probiotic preparation. In addition, the preparation method of the present invention can more conveniently realize the large-scale production of probiotic microencapsulated products, and is suitable for the preparation of probiotic microcapsules in various fields such as feed, food, and medicine.
Description
technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a preparation method of multi-layer coated probiotic microcapsules. Background technique [0002] The function of live probiotic preparations mainly depends on the life activities of the live bacteria contained in them. Therefore, the content of live bacteria, the vitality of live bacteria and the length of shelf life are the key factors to evaluate the quality of products. At present, researchers at home and abroad regard maintaining the survival and stability of probiotic preparations as the standard for quality evaluation. In order to solve the problem of low survival rate and instability of probiotics in use, the use of microcapsule embedding technology to protect probiotics has become a hot research topic at home and abroad. However, most of the existing microcapsule coating technologies and products have the problem of surface porosity, especially t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More