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Rose active extract and application thereof

A technology of extract and flower activity, applied in the field of rose flower active extract

Active Publication Date: 2021-04-06
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early days, sulfur-containing compounds were widely used in the field of food preservation, but their use was restricted due to certain negative effects of sulfur residues on human health

Method used

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  • Rose active extract and application thereof
  • Rose active extract and application thereof
  • Rose active extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation and chemical composition analysis of different rose flower crude extracts of embodiment 1

[0026] Take 50g of dried petals of each rose flower (Chuanyun rose, silk rose, Emei rose, northwest rose, multi-bud rose and yellow rose collected in the wild), crush them and put them in different triangular flasks, add 500mL 50% Ethanol aqueous solution, extracted at 50°C for 10 hours, filtered to obtain each rose flower extract, and the extracts were respectively concentrated under reduced pressure at 50°C to obtain each rose flower crude extract, which were named CDQWH-1A, JMQWH-1A, EMQWH in turn -1A, XBQWH-1A, DBQWH-1A and HQWH-1A. Taking the separated Gemin A and Gemin B as reference substances, the above-mentioned 6 kinds of rose crude extracts were analyzed by reversed-phase liquid chromatography, and the mass fractions of Gemin A and Gemin B in the multi-bud rose crude extracts were 1.1 % and 0.2%, Gemin A and Gemin B were not detected in the other 5 species...

Embodiment 2

[0028] Grind 50g of rose multi-bud, soak in 10 times the amount of 50% ethanol aqueous solution at 50°C for 10 hours, soak for 3 times in total, combine the extracts, concentrate and dry under reduced pressure at 50°C to obtain 16.8g of extract. The extract is suspended in water, first extracted three times with petroleum ether-ethyl acetate (volume ratio 1:1) mixed solvent to remove the fat-soluble components, then extracted three times with n-butanol, combined n-butanol extracts, Concentrate under reduced pressure at 50°C to obtain 5.1 g of n-butanol extract. The n-butanol extract was dissolved in water and passed through HPD-100A macroporous adsorption resin, eluted with pure water for 10 column volumes, then eluted with 25% ethanol aqueous solution for 10 column volumes, and the eluents of 25% ethanol aqueous solution were combined , concentrated under reduced pressure at 50°C to obtain 2.3 g of an active extract rich in target active ingredients; the active extract was su...

Embodiment 3

[0036] Grind 200g of Rosa multibud, extract with 10 times the amount of 35% ethanol aqueous solution at 50°C for 10 hours, extract three times, combine the extracts, concentrate under reduced pressure at 40°C to obtain 62g of extract. Dissolve the extract in 2L of pure water, pass through the HPD-100A macroporous adsorption resin, first elute with pure water for 8 column volumes, then elute with 30% ethanol aqueous solution for 10 column volumes, and combine 30% ethanol for elution The solution was concentrated under reduced pressure at 45°C until the solution had no obvious alcohol taste, and the solution was frozen and then vacuum-dried at low temperature to obtain 9.1 g of active extract (code DBQWH-1). Using the separated Gemin A and Gemin B as reference substances, the active extract was analyzed by reversed-phase liquid chromatography, the mass fraction of Gemin A was 5.2%, and the mass fraction of Gemin B was 1.1%.

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Abstract

The invention relates to the technical field of skin care products, in particular to a rose active extract and application thereof. According to the specific technical scheme, the rose active extract contains Gemin A and / or Gemin B as active ingredients, the mass percentage of Gemin A is larger than or equal to 4.0%, and / or the mass percentage of Gemin B is larger than or equal to 0.8%. Active ingredients have good tyrosinase inhibition activity and can be used as tyrosinase inhibitors in whitening skin care products and fruit and vegetable preservatives. Gemin A, and / or Gemin B or rosa multibracteata can also be added into the tyrosinase inhibitor to be used as an active ingredient.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to an active extract of rose flower and its application. Background technique [0002] It is well known that excessive ultraviolet radiation will cause the human body to produce a large number of free radicals and reactive oxygen species, which in turn will cause oxidative damage to the body. Excessive active oxygen in the human body will promote tyrosinase to catalyze L-tyrosine to undergo a series of complex reactions and finally form melanin. An appropriate amount of melanin can protect personal safety, but a large amount of active oxygen in the human body will cause pigment spots and malignant diseases such as melanoma. Therefore, tyrosinase inhibitors have good application potential in fields such as medicine and whitening cosmetics. [0003] At present, with the continuous improvement of material living standards, people pay more attention to skin care and put for...

Claims

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Application Information

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IPC IPC(8): A61K8/60A61Q19/02A23B7/154
CPCA61K8/602A61Q19/02A23B7/154A61K2800/782A61K2800/805A23V2002/00A23V2250/21
Inventor 李甫王明奎王伦陈斌
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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