Multichannel organophosphorus pesticide rapid detector based on Internet of Things and detection method
A technology of organophosphorus pesticides and detection methods, applied in the field of electrochemical sensing and detection, can solve the problems of difficult on-site rapid detection, complex equipment structure, high cost, etc., achieve high promotion and application value, accurate detection results, and easy to carry Effect
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Embodiment 1
[0046] Example 1 Preparation of a microfluidic fixture combined with an array electrode chip:
[0047] (1) PDMS chip production: Use SolidWorks software to draw the basic graphics of the microfluidic fixture, use a high-resolution laser phototypesetter to draw a photolithography mask on the photographic film, and then spin-coat SU-8 photoresist on the silicon wafer layer, perform high-temperature curing treatment on it, cover the photolithography mask and expose the cured SU-8 photoresist on the photolithography machine, and finally develop it in the developer to obtain the silicon wafer positive mold template;
[0048] (2) PDMS prepolymer and PDMS curing agent are mixed at a ratio of 10:1, and poured on the prepared male template after pumping in a vacuum, and peeled off after high temperature curing to obtain a chip with a microstructure, according to the drawing Drill out the bottom liquid filling hole and the bottom liquid discharge hole;
[0049] (3) Fix and seal the arr...
Embodiment 2
[0050] Example 2 Detection of organophosphorus pesticides by array electrode chip combined with microfluidic fixture
[0051] (1) Wash the channel with PBS before sample injection;
[0052] (2) Sampling: Take 50 μL of the bottom liquid of the sample, and inject it into the injection hole through the injection diaphragm pump.
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