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Multichannel organophosphorus pesticide rapid detector based on Internet of Things and detection method

A technology of organophosphorus pesticides and detection methods, applied in the field of electrochemical sensing and detection, can solve the problems of difficult on-site rapid detection, complex equipment structure, high cost, etc., achieve high promotion and application value, accurate detection results, and easy to carry Effect

Pending Publication Date: 2021-04-09
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Food detectors can quickly detect pesticide residues in various foods (agricultural products), and are widely used in the detection of vegetables, fruits, medicinal materials, aquatic products, meat products, pickles, etc. The application of electrochemical biosensors to the monitoring of pesticide residues has caused In various pesticide residue detection technologies, classic technologies such as large-scale gas and liquid chromatography used in laboratories have high detection accuracy, but the operation is complicated, the cost is high, and it is difficult to achieve rapid on-site detection; and Existing rapid detection products often have problems such as low precision, complex equipment structure, and difficult operation. Compared with traditional high-performance liquid chromatography, gas chromatography, and other laboratory detection methods, electrochemical biosensors have fast detection speed and easy operation. It has the advantages of low price and high sensitivity; and the pesticide residue detector made of biosensor is easy to carry after miniaturization, and it is convenient for field detection outdoors

Method used

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  • Multichannel organophosphorus pesticide rapid detector based on Internet of Things and detection method
  • Multichannel organophosphorus pesticide rapid detector based on Internet of Things and detection method
  • Multichannel organophosphorus pesticide rapid detector based on Internet of Things and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of a microfluidic fixture combined with an array electrode chip:

[0047] (1) PDMS chip production: Use SolidWorks software to draw the basic graphics of the microfluidic fixture, use a high-resolution laser phototypesetter to draw a photolithography mask on the photographic film, and then spin-coat SU-8 photoresist on the silicon wafer layer, perform high-temperature curing treatment on it, cover the photolithography mask and expose the cured SU-8 photoresist on the photolithography machine, and finally develop it in the developer to obtain the silicon wafer positive mold template;

[0048] (2) PDMS prepolymer and PDMS curing agent are mixed at a ratio of 10:1, and poured on the prepared male template after pumping in a vacuum, and peeled off after high temperature curing to obtain a chip with a microstructure, according to the drawing Drill out the bottom liquid filling hole and the bottom liquid discharge hole;

[0049] (3) Fix and seal the arr...

Embodiment 2

[0050] Example 2 Detection of organophosphorus pesticides by array electrode chip combined with microfluidic fixture

[0051] (1) Wash the channel with PBS before sample injection;

[0052] (2) Sampling: Take 50 μL of the bottom liquid of the sample, and inject it into the injection hole through the injection diaphragm pump.

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Abstract

The invention relates to the field of electrochemical sensing detection, in particular to a multichannel organophosphorus pesticide rapid detector based on the Internet of Things, preparation of an array electrode combined with an array microfluidic clamp and a detection method of the array electrode. The pesticide detector is characterized in that a potentiostat is used as a core, a multi-channel microfluidic clamp is used as sample injection equipment, a microcontroller is used as a control center of the rapid detector, an HMI serial port screen is used as a display channel of a lower computer, the array electrode is used as an electrode of an organophosphorus pesticide electrochemical biosensor; a multichannel relay is used for controlling a sample injection diaphragm pump to inject an actual sample, RFID is used as an acquisition module of employee card information, acquired multichannel digital signals are transmitted to a cloud server through a communication module by MQTT and an HTTP protocol, the acquired multichannel digital signals are forwarded to an MQTT main server after summarization is finished, the MQTT main server triggers a storage rule to transfer MYSQL, data is displayed and queried by using an application layer built by django + vue, the data is processed and stored, and a test report is generated.

Description

technical field [0001] The present invention relates to the field of electrochemical sensing and detection, in particular to a multi-channel rapid organophosphorus pesticide detector based on the Internet of Things, and specifically to the preparation of an array electrode combined with an array microfluidic fixture and the construction of a detection method . Background technique [0002] Food detectors can quickly detect pesticide residues in various foods (agricultural products), and are widely used in the detection of vegetables, fruits, medicinal materials, aquatic products, meat products, pickles, etc. The application of electrochemical biosensors to the monitoring of pesticide residues has caused In various pesticide residue detection technologies, classic technologies such as large-scale gas and liquid chromatographs used in laboratories have high detection accuracy, but the operation is complicated, the cost is high, and it is difficult to achieve rapid on-site dete...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30B01L3/00
CPCG01N27/48G01N27/30B01L3/5027
Inventor 郭业民项耀东徐睿管福凯齐广宇
Owner SHANDONG UNIV OF TECH
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