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A group of piRNA biomarkers for early diagnosis of breast cancer and its application

A biomarker and breast cancer technology, applied in the fields of molecular biology and oncology, can solve the problems of unclear diagnostic value of expression level and achieve the effect of making up for poor specificity

Active Publication Date: 2022-02-01
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression levels of circulating piRNAs in breast cancer patients and their diagnostic value in breast cancer remain unclear

Method used

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  • A group of piRNA biomarkers for early diagnosis of breast cancer and its application
  • A group of piRNA biomarkers for early diagnosis of breast cancer and its application
  • A group of piRNA biomarkers for early diagnosis of breast cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 qRT-PCR detection of piR651, piR17458 and piR20485 levels in serum of breast cancer patients

[0019] Sera from breast cancer patients and healthy individuals were obtained from the Third Affiliated Hospital of Guangzhou Medical University. Serum was placed in a high-speed refrigerated centrifuge at 10,000 rpm for 10 minutes to remove cell debris. RNA was extracted using miRNeasy Serum / Plasma Kit from QIAGEN Company, miScript II RT Kit was used for reverse transcription, and real-time fluorescent PCR experiments were performed with miScript SYBR Green PCR Kit.

[0020] PCR reaction system: 10 μl of 2X SYBR Green PCR mixture, 2 μl of universal primers, 4 μl of RNase free water, 2 μl of cDNA template and 2 μl of corresponding specific piRNA primers. The specific primer sequences are shown in Table 1.

[0021] PCR reaction program: 95°C for 15min; 94°C for 15s, 55°C for 30s, 70°C for 30s, a total of 40 amplification cycles.

[0022] Table 1 qRT-PCR primers

...

Embodiment 2

[0025] Example 2 ROC curve analysis of the application value of the combination of piR651, piR17458 and piR20485 in serum in the diagnosis of breast cancer

[0026] SPSS17.0 software was used to analyze the application value of the combination of piR651, piR17458 and piR20485 in the diagnosis of breast cancer. The result is as figure 2 As shown, the ROC curve analysis results show that the combination of piR651, piR17458 and piR20485 in the diagnosis of breast cancer has an AUC area of ​​0.8113, indicating that the combination of serum piR651, piR17458 and piR20485 has a good clinical value for the diagnosis of breast cancer; at the same time, the ROC curve analysis It shows that the sensitivity and specificity of the combination of piR651, piR17458 and piR20485 in the diagnosis of breast cancer are 67.7% and 84.8%, respectively; the 95% confidence interval is 0.3-0.7, which has the characteristics of good specificity and high sensitivity.

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Abstract

The present invention discloses a group of piRNA biomarkers for breast cancer diagnosis and its application, and belongs to the fields of molecular biology and oncology. The piRNA biomarkers are piR651, piR17458 and piR20485; wherein, the nucleotide sequence of piR651 is as SEQ ID As shown in NO:1, the nucleotide sequence of piR17458 is shown in SEQ ID NO:2; the nucleotide sequence of piR20485 is shown in SEQ ID NO:3. The present invention finds that the combination of piR651, piR17458 and piR20485 can be used as breast cancer diagnostic markers and applied to breast cancer screening, especially early breast cancer screening, to make up for the current breast cancer diagnostic markers and early breast cancer diagnostic markers The specificity of the substance is not good, and the sensitivity is not enough.

Description

technical field [0001] The invention relates to the fields of molecular biology and oncology, in particular to a group of piRNA biomarkers for early diagnosis of breast cancer and applications thereof. Background technique [0002] Breast cancer is the most common malignant tumor in women and one of the most lethal tumors. The cause of death from breast cancer is not due to the growth of carcinoma in situ itself, but distant metastasis. Once breast cancer metastasizes, its five-year survival rate is only 23%. 70-80% of breast cancer patients found in our country are patients with advanced tumors. Breast cancer has become an urgent public health problem and a hotspot of clinical research. Studies have shown that early diagnosis and surgical resection of breast cancer can effectively improve the quality of life, survival and prognosis of patients. At present, the diagnosis of breast cancer includes serum markers, pathological diagnosis, imaging and clinical symptoms, but t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 洪宏海尹萍夏勇
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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