A Lactobacillus acidophilus producing exopolysaccharide with antitumor and hypoglycemic and lipid-lowering activities and its application

A technology of Lactobacillus acidophilus, hypoglycemic and lipid-lowering, applied in the field of Lactobacillus acidophilus of extracellular polysaccharides, can solve the problem that no patents of Lactobacillus acidophilus have been retrieved, separation and purification, anti-tumor, hypoglycemic and weight loss. Patent and other issues, to achieve the effect of inhibiting the proliferation of colon cancer and cervical cancer cells, good gastrointestinal environment tolerance, anti-tumor, hypoglycemic and lipid-lowering activities

Active Publication Date: 2022-07-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In a word, in the published patents or patent applications, although Lactobacillus acidophilus has been reported to improve blood sugar, weight loss and lipid lowering, no patents have been retrieved on the anti-colon and cervical cancer of Lactobacillus acidophilus. There are no related patents on the separation and purification of exopolysaccharides of Lactobacillus, and research on anti-tumor, hypoglycemic and weight loss

Method used

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  • A Lactobacillus acidophilus producing exopolysaccharide with antitumor and hypoglycemic and lipid-lowering activities and its application
  • A Lactobacillus acidophilus producing exopolysaccharide with antitumor and hypoglycemic and lipid-lowering activities and its application
  • A Lactobacillus acidophilus producing exopolysaccharide with antitumor and hypoglycemic and lipid-lowering activities and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1: Isolation, purification and identification of strains

[0083] (1) Isolation and purification of strains

[0084] Take the yogurt from the Hetao area as a sample and dilute it to 10 times with sterile normal saline. -6 , take 10 -4 , 10 -5 , 10 -6 100 μl of the three gradient dilutions were spread on MRS solid plates, cultured at 37°C for 48 h, and colonies of different shapes were selected for streaking until a pure single colony with the same colony shape was obtained, and a single colony was picked and inoculated into 5 ml of MRS liquid. In the medium, cultured overnight, take 800 μl of bacterial liquid, add 200 μl of 60% glycerol, and store at -80°C.

[0085] (2) Identification of strains

[0086] The isolated strains were subjected to PCR amplification of 16S rDNA, and the PCR products were sent to Jinweizhi for sequencing. The sequencing results were compared in EzBioCloud, and the similarity was 91.29%. One strain of Lactobacillus acidophilus was ...

Embodiment 2

[0089] Example 2: Growth curves of strains

[0090] The Lactobacillus acidophilus in Example 1 was inserted into MRS solid medium and cultured anaerobically at 37°C for 48h. Pick a single colony with good growth status and inoculate it into MRS liquid medium for activation, and then inoculate it in MRS liquid medium with 1% inoculum, let it stand for 24 hours, and measure the OD600 of the bacterial suspension every hour, and draw the Growth curve of Lactobacillus acidophilus. like figure 1 shown.

Embodiment 3

[0091] Example 3: Determination of acid resistance of Lactobacillus acidophilus YL01

[0092] The lactic acid bacteria were activated three times, placed in an anaerobic incubator at 37 °C, and cultured overnight; the concentration of the bacteria was diluted to 5 × 10 8 CFU / mL, replaced with MRS medium with different pH values, pH was 2.0, 3.0, 4.0; bacterial solutions with different pH values ​​were placed in 96-well plates, each experimental group was set with 6 parallel wells, and a negative control needs to be set , and its negative control was MRS with pH 7, which was placed in an anaerobic incubator at 37 °C for 4 h. 20 μL of MTT (5 mg / mL) will be added and placed in an anaerobic incubator for 4 h. The absorbance value of each well at 490nm (A experimental group) was measured in an enzyme-linked immunosorbent assay instrument, and compared with the absorbance value of the negative control group (A control group) to determine the survival rate. The formula for calculat...

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Abstract

The invention discloses a Lactobacillus acidophilus with anti-tumor and hypoglycemic and lipid-lowering activities, the name is: YL01, the classification name is: Lactobacillus acidophilus, the preservation number is: CGMCC No.20085, the preservation date: 2020 On June 15th, the preservation unit is: General Microbiology Center of China Microbial Culture Collection Management Committee, Institute of Biology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The Lactobacillus acidophilus YL01 of the invention has good acid and bile salt tolerance, can alleviate diet-induced hyperlipidemia, alleviate the weight gain of mice induced by high-fat diet, significantly reduce postprandial blood glucose levels, and enhance the body's ability to respond to glucose tolerance, reducing insulin resistance. The Lactobacillus acidophilus of the invention can be used for preparing fermented food, and has a very wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of polysaccharides in microbiology, and relates to a microbial extracellular polysaccharide, in particular to a Lactobacillus acidophilus producing extracellular polysaccharide with anti-tumor, hypoglycemic and lipid-lowering activities, and applications thereof. Background technique [0002] Lactic acidbacteria (LAB) exopolysaccharides (EPS) are secondary metabolites secreted outside the cell wall by lactic acid bacteria during growth and metabolism. According to their ability to bind to the cell wall, they can be divided into two types: one. Loss of the ability to combine with cells and infiltrate into the medium to form mucus, called mucopolysaccharide; another adheres to the cell wall to form a capsule, called capsular polysaccharide. Most of the EPS-producing strains reported so far produce mucopolysaccharides, while some LAB strains can produce both capsular polysaccharides and mucopolysaccharides. La...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04A23C9/123A23C9/13A23L11/50A23L19/00A23L29/00A23L33/135A23L33/125A23L2/38A23L2/84A23L2/52A23G3/36A23G3/42A61K35/747A61P35/00A61P3/04C12R1/23
CPCY02A50/30
Inventor 王楠张同存申敬杨在明赵崇杰盖塞伦齐威罗学刚
Owner TIANJIN UNIV OF SCI & TECH
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