Anti-aging protein polypeptide and preparation method thereof
A protein peptide, anti-aging technology, applied in the field of health care products, to achieve the effect of improving the effect of eating, good activity, and excellent free radical scavenging effect
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Embodiment 1
[0026] A method for preparing an anti-aging protein polypeptide, comprising the steps of:
[0027] (1) Raw material premixing treatment:
[0028] After the sea cucumber is pulverized, it is blended with royal jelly, and after stirring at a high speed, the mixed raw material is obtained for later use;
[0029] (2) Enzymolysis treatment:
[0030] Put the mixed primary material obtained in step (1) into the tank, and then successively undergo hydrolysis with pepsin, trypsin, and papain to obtain an enzymatic hydrolysis solution for subsequent use;
[0031] (3) Precipitation treatment:
[0032] Adding ethanol to the enzymolysis solution obtained in step (2) for precipitation, and centrifuging after standing for 8 hours, and taking the supernatant for subsequent use;
[0033] (4) Filtration and drying treatment:
[0034] The supernatant obtained in step (3) is subjected to preliminary filtration treatment, then ultrafiltration treatment, then adding activated carbon for decolor...
Embodiment 2
[0045] A method for preparing an anti-aging protein polypeptide, comprising the steps of:
[0046] (1) Raw material premixing treatment:
[0047] After the sea cucumber is pulverized, it is blended with royal jelly, and after stirring at a high speed, the mixed raw material is obtained for later use;
[0048] (2) Enzymolysis treatment:
[0049] Put the mixed primary material obtained in step (1) into the tank, and then successively undergo hydrolysis with pepsin, trypsin, and papain to obtain an enzymatic hydrolysis solution for subsequent use;
[0050] (3) Precipitation treatment:
[0051] Add ethanol to the enzymolysis solution obtained in step (2) for precipitation, and finally leave it to stand for 10 hours and then centrifuge, and take the supernatant for subsequent use;
[0052] (4) Filtration and drying treatment:
[0053] The supernatant obtained in step (3) is firstly filtered, then ultrafiltered, then activated carbon is added for decolorization, and finally it i...
Embodiment 3
[0064] A method for preparing an anti-aging protein polypeptide, comprising the steps of:
[0065] (1) Raw material premixing treatment:
[0066] After the sea cucumber is pulverized, it is blended with royal jelly, and after stirring at a high speed, the mixed raw material is obtained for later use;
[0067] (2) Enzymolysis treatment:
[0068]Put the mixed primary material obtained in step (1) into the tank, and then successively undergo hydrolysis with pepsin, trypsin, and papain to obtain an enzymatic hydrolysis solution for subsequent use;
[0069] (3) Precipitation treatment:
[0070] Add ethanol to the enzymolysis solution obtained in step (2) for precipitation, and finally leave it to stand for 12 hours and then centrifuge, and take the supernatant for subsequent use;
[0071] (4) Filtration and drying treatment:
[0072] The supernatant obtained in step (3) is subjected to preliminary filtration treatment, then ultrafiltration treatment, then adding activated carbo...
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