Stachyurus extract and application thereof
The technology of the flower and the extract is applied to the extract of the flower and its application in cosmetics to achieve the effect of inhibiting the activity of tyrosinase, good effect and inhibiting the production of melanin
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Embodiment 1
[0036] Example 1 Alcoholic extract of Jingjiehua
[0037] Dried the stem pith of Himalayan japonica until the water content is ≤5%, take 100g, grind it into a coarse powder with a particle size of 25-800 mesh, put it in 4L of 70% (v / v) ethanol aqueous solution, and extract it with 500W ultrasonic wave at room temperature for 1h;
[0038] The solid was collected by filtration, and the above extraction step was repeated once.
[0039] The filtrates were combined and evaporated to dryness at 40-60° C. to obtain about 2 g of ethanol extract of Himalayan japonica.
Embodiment 2
[0040] Example 2 The water extract of Jingjiehua
[0041] Dry the stem pith of Himalaya japonica until the water content is ≤5%, take 100g, grind it into a coarse powder with a particle size of 25-800, put it in 6L of deionized water, heat it in a water bath at 96°C for 1 hour, and separate the solid and filtrate by suction filtration;
[0042] Take the solid and repeat the above extraction step once.
[0043] The filtrates were combined, concentrated and freeze-dried to obtain about 2 g of the water extract of Himalayan Jasmine Flower.
Embodiment 3
[0044] Example 3 DPPH method scavenging free radical activity test
[0045] 1,1-Diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl, DPPH) is a stable nitrogen-centered organic radical, and its ethanol solution is dark purple at about 517nm There is strong absorption; when there is a free radical scavenger, the absorption of DPPH is weakened and gradually disappears due to its single-electron pairing. The degree of fading of DPPH ethanol solution has a quantitative relationship with the number of electrons it accepts. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect free radical scavenging and evaluate the ability of antioxidants to scavenge free radicals, antioxidant capacity and anti-aging capacity.
[0046] Preparation of DPPH ethanol solution: Accurately weigh 6mg of DPPH, dissolve it with 95% ethanol and set the volume in a 50mL volumetric flask, and dissolve with the aid of ultrasound. When used, dilute it four times wit...
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