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Segmented enzymolysis process of plukenetia volubilis linneo, and plukenetia volubilis linneo peptide prepared by same

The technology of meridian fruit and enzymolysis, applied in the field of meridian fruit peptide, can solve the problems of difficult to control fermentation process, complicated preparation process, complicated operation, etc., and achieve the effect of improving peptide yield, uniform molecular weight and high purity.

Pending Publication Date: 2021-07-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the patent CN110066844A, a method for preparing bioactive peptides of satobi fruit meal with the effect of reducing uric acid also proposes a method for preparing satoko fruit active peptides. This method requires lactobacillus to ferment, and the operation is complicated and takes a long time. The fermentation process is difficult to control
Therefore, in order to solve the complex problem in the preparation process of satosa bioactive peptides in reality, this patent proposes a method for preparing satosa bioactive peptides by a hierarchical enzymatic hydrolysis process

Method used

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  • Segmented enzymolysis process of plukenetia volubilis linneo, and plukenetia volubilis linneo peptide prepared by same
  • Segmented enzymolysis process of plukenetia volubilis linneo, and plukenetia volubilis linneo peptide prepared by same
  • Segmented enzymolysis process of plukenetia volubilis linneo, and plukenetia volubilis linneo peptide prepared by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A segmented enzymatic hydrolysis process of saiteng fruit, comprising the steps of:

[0033] S1. Raw material pretreatment: pulverize the cane fruit cake, and then pass through a 100-mesh sieve to obtain the cane fruit meal powder for later use;

[0034] S2. High-temperature treatment: add sachaeji fruit meal powder into pure water according to the material-to-liquid ratio of 1:20 (w / v), heat it in a water bath at 95°C, and keep it warm for 6 hours;

[0035] S3. Proteolysis: After high-temperature denaturation of cane fruit protein, take it out and cool it to about 50°C, add 0.75% alkaline protease, enzymolyze it at pH 10 and 60°C for 6 hours, centrifuge after the enzymolysis is completed, and collect the solution A;

[0036] S4. Secondary enzymatic hydrolysis: Add pure water to the sediment according to the ratio of material to liquid 1:10 (w / v), add 0.25% neutral protease, and enzymolyze at pH 7 and 50°C for 3 hours. After the enzymolysis is completed Perform centri...

Embodiment 2

[0040] A segmented enzymatic hydrolysis process of saiteng fruit, comprising the steps of:

[0041] S1. Raw material pretreatment: pulverize the cane fruit cake, and then pass through a 100-mesh sieve to obtain the cane fruit meal powder for later use;

[0042] S2. High-temperature treatment: add sachaeji fruit meal powder into pure water according to the material-to-liquid ratio of 1:30 (w / v), heat in a water bath at 100°C, and keep warm for 4 hours;

[0043] S3. Proteolysis: After high-temperature denaturation of the cane fruit protein, take it out and cool it to about 50°C, add 0.9% alkaline protease, and enzymolyze it at pH 9 and 50°C for 3 hours, centrifuge after the enzymolysis is completed, and collect the solution A;

[0044] S4. Secondary enzymatic hydrolysis: add pure water to the sediment according to the ratio of material to liquid 1:15 (w / v), add 0.4% neutral protease, and enzymolyze at pH 7 and 50°C for 3 hours, after the enzymolysis is completed Perform centri...

experiment example 1

[0055] Determination of the yield of the polypeptide of the United Teng fruit:

[0056] Take 5 mL of enzymatic hydrolysis supernatant to measure its protein content; take another 5 mL of enzymatic hydrolysis supernatant, add 5 mL of 20% trichloroacetic acid solution, shake well, let stand for 30 min, centrifuge at 4000 r / min for 20 min, and take supernatant to determine peptide yield.

[0057] Take respectively implementation case 1, implementation case 2, and the prepared satocarpine samples of comparison case 1, and measure its protein content; after taking another batch of samples to make a solution, add 5mL20% trichloroacetic acid solution, shake well, Let it stand for 30 minutes, centrifuge at 4000r / min for 20 minutes, take the supernatant to measure the yield of polypeptide, the calculation formula is:

[0058] Peptide yield = (acid-soluble protein content after hydrolysis - acid-soluble protein content before hydrolysis) / total protein content × 100%

[0059] The resu...

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Abstract

The invention discloses a segmented enzymolysis process of plukenetia volubilis linneo, and plukenetia volubilis linneo peptide prepared by the same, and belongs to the technical field of active peptide production. The segmented enzymolysis process of the plukenetia volubilis linneo comprises the steps of raw material pretreatment, high-temperature treatment, proteolysis, secondary enzymolysis, enzyme deactivation, concentration and drying. The process is simple to operate, mild in treatment condition and high in polypeptide yield, the sacha inchi protein is hydrolyzed by adopting a method combining high-temperature cooking and enzymolysis, the space structure of the protein is changed by high temperature, the space structure is loose, the protein is more easily hydrolyzed by protease, the polypeptide yield is obviously improved, and the plukenetia volubilis linneo peptide prepared by the process is low and uniform in molecular weight and relatively high in purity, can quickly penetrate through the oral cavity and the stomach of a human body to directly enter the small intestine, can be better digested and absorbed by the human body, has the effect of remarkably scavenging free radicals, and can be applied to the field of health care products as a natural antioxidant.

Description

technical field [0001] The invention belongs to the technical field of active peptide production, and relates to a staged enzymatic hydrolysis process of satoshi fruit and satoko peptide prepared therefrom. Background technique [0002] The scientific name of American vine fruit is South American oil vine, which is a perennial woody vine of Euphorbiaceae. At present, it is mainly planted in many countries and regions such as Myanmar, Laos, Yunnan Pu'er, Xishuangbanna, etc., and the planting scale is gradually expanding. At present, the seeds of cane fruit are mainly used for the extraction of oil, and a large amount of cake will be produced accordingly. The seed kernel of Meiteng fruit contains 27% protein, which is second only to soybean, and the protein content in the cake is more than 60%. Studies have found that the nutritional value of cane fruit protein is high, and it is a high-quality source of plant protein, rich in 9 kinds of essential amino acids. With the cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
CPCC12P21/06C07K1/14
Inventor 黎攀黄思苑杜冰林锦铭温嘉敏李俊健
Owner SOUTH CHINA AGRI UNIV
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