A method for screening maize high temperature resistant materials based on flowering characteristics
A feature screening and heat-resistant technology, applied in botany equipment and methods, angiosperms/flowering plants, plant genetic improvement, etc., can solve problems such as yield impact, difficult-to-heat-resistant corn materials, grain abortion and grain number reduction, etc. Achieve the effects of high accuracy and efficiency, solve the difficulty of screening and identification, and shorten the time and cost
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Embodiment 1
[0022] Example 1 Correlation analysis between flowering characteristics and seed setting rate under high temperature environment
[0023] In 2019, the corn material was sown at the end of May, and 2 replicates were set up, arranged in random blocks, and 20 plants were planted in each replicate. The flowering period of the material is between the end of July and the first ten days of August, and the average daily maximum temperature during this period is 35.6℃. Correlation analysis was carried out on the flowering characteristics of 20 inbred lines (Table 1) under the high temperature treatment at the sowing date and the self-seeding rate of the materials. Pollen vigor (correlation coefficient 0.59) was significantly positively correlated, significantly negatively correlated with loose powder spinning interval (correlation coefficient -0.53), and other traits were not significantly correlated (Table 2).
[0024] Table 1 Materials and sources of 20 inbred lines used in the test...
Embodiment 2
[0028] Embodiment 2 Screening of flowering characteristic index under high temperature environment
[0029] The flowering characteristics obtained in Example 1 with a high correlation with the self-seeding rate were selected to further determine the indicators of each characteristic.
[0030] From the perspective of yield reduction under high temperature stress, the number of grains per ear was selected as the final reference index for breeding. Therefore, the self-seeding rate ≥ 75% was used as the screening standard for high temperature resistant materials. If the standard is too high, it is difficult to select the materials that meet the standard. If the standard is too low, the production reduction will be too serious, and there will be no value in production. The establishment of this indicator also takes into account the risk of yield reduction that can be endured by field-produced varieties under adversity stress and the scarcity of high-temperature resistant materials...
Embodiment 3
[0043] Example 3 The heat resistance identification of 210 DH series materials using the screening index of Kaihua characteristics
[0044]On June 25, 2020, 210 corn DH lines (DH lines are pure lines generated by haploid breeding technology) were planted. The specific information and sources are shown in Table 7. And these DH series materials were identified for high temperature resistance, and the tasseling time of 210 materials was from August 10th to August 23rd. During this period, the average daily maximum temperature at the test station was 35.2 °C. The pollen viability, the rate of bracts spun out of filaments and the interval of loose filaments spun out of these materials were counted. According to the identification criteria of Kaihua characteristics determined in Example 1 and Example 2 (pollen vigor ≥ 50%, bract spit rate ≥ 75%, and loose powder spinning interval ≤ 2 days), preliminarily judged heat-resistant materials, a total of 26 copies , see Table 8 for detai...
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