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Specific primers and applications of molecular markers closely related to fruit shape index

A related molecular marker and specific technology, applied in the field of molecular biology, can solve the problems of undiscovered genes that control fruit shape, and undetermined genes that control the contribution rate of fruit shape index.

Active Publication Date: 2022-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the existing research, it is proved that the fruit shape index is a quantitative trait genetic locus. Although many QTLs related to fruit shape have been mapped, the genes controlling fruit shape have not been discovered, let alone the contribution to the control of fruit shape index. high rate gene

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  • Specific primers and applications of molecular markers closely related to fruit shape index
  • Specific primers and applications of molecular markers closely related to fruit shape index

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Embodiment 1

[0015] 311 annual capsicum (Capsicum annuum L.) leaves were accessed, and the samples were sent to the company to extract DNA for resequencing. The sequencing platform was Illumina Solexa, and the amount of sequencing data for each material was about 30G. Trimmomatic v0.33 was used to process the original data and remove the Linkers, poly-N and low-quality fragments were used to obtain clean data, and BWA0.75 was used to align the clean data to the Zunla-1 genome data (Qin et al., 2014), and the parameters were set to aln-o 1-e 10-t 4 -l 32-i 15-q 10; use Samtools 0.1.19 (Li et al., 2009), use the Bayesian algorithm, set the parameters -q 1-C50-S-D-m 2-F 0.002-u, run the command mpileup to find SNPs , filter SNPs, set the minimum gene allele frequency greater than 0.01, and the deletion rate less than 0.1.

[0016] Phenotypic identification of the fruit shape index of 311 existing annual pepper materials was carried out. The fruit shape index was the ratio of the length of the...

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Abstract

The invention relates to the field of molecular biology, in particular to specific primers and applications of molecular markers closely related to fruit shape index. The present invention determines a SNP that is significantly related to fruit shape, and determines that it is a candidate gene for controlling the fruit shape index of pepper. By designing amplification and sequencing primers, PCR amplification is performed on the segment containing the SNP, and the PCR amplification is carried out. The amplified products were subjected to Sanger sequencing, and the genotype of the SNP site was identified according to the sequencing results.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to specific primers and applications of molecular markers closely related to fruit shape indices. Background technique [0002] Capsicum (Capsicum spp.) is a crop of Capsicum (Capsicum) of the family Solanaceae, native to Mexico and Colombia, and widely cultivated in countries around the world. Different chili varieties with similar flavors and good fruit shape can attract consumers more and have more price advantages in the market, so as to achieve the purpose of increasing profits and income. At the same time, as an ornamental variety of pepper, the fruit shape is an important commodity quality of pepper. With the continuous expansion of my country's pepper market and the continuous increase of planting scale, genetic research on pepper fruit shape has become more and more important. The fruit shape of pepper is a highly differentiated trait, and there are many shapes of pepper ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A50/30
Inventor 王立浩曹亚从程锋张亢于海龙张宝玺张正海景雅欣靳远张伟丽陈姝敏
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI