Method for increasing survival rate of hybrid offspring seedlings of black poplar

A survival rate and progeny technology, applied in the field of plant seedlings, can solve the problems of tissue cultured seedlings being easily damaged, long seedling slowing period, and low seedling rate of hybrid progeny tissue cultured seedlings, so as to improve the survival rate of transplanting, Effect on reducing root damage

Active Publication Date: 2021-06-01
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not easy to solve the sterility, ventilation, and nutritional requirements for the root growth of tissue cultured seedlings at the same time when the above method is used for indoor hardening of seedlings. In addition, during the process from indoor hardening to field transplanting, the roots of tissue cultured seedlings are easily damaged. Longer, resulting in low seedling rate of hybrid progeny tissue culture seedlings

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  • Method for increasing survival rate of hybrid offspring seedlings of black poplar

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Embodiment 1

[0025] The invention provides a seedling hardening and transplanting method of poplar tissue culture seedlings to improve the seedling rate of poplar hybrid seedlings, which is specifically achieved by the following contents:

[0026] 1) 30 days after pollination, cut off the capsule from the base of the fruit stalk with scissors, rinse the surface of the pericarp with tap water, drain the water, and put it into a sterile bottle. Soak and disinfect with 75% alcohol for 6 minutes, then rinse with sterile water three times, put the material into a petri dish after rinsing, and blot the moisture on the surface of the material with sterile filter paper.

[0027] 2) Gently press with a blade along the longitudinal axis of the carpel, peel off the pericarp, gently peel off the floc and seed embryo, inoculate evenly on 1 / 2MS medium, and press lightly with an inoculation tool to fully contact the medium, Good for germination and growth. The culture environment temperature is 25°C±2°C...

Embodiment 2

[0033] The invention provides a seedling hardening and transplanting method for hybrid embryo tissue culture seedlings of Zhongqian No. 3 × Juba Yang, Danhong Yang × Zhonghan 17, Zhongzhu No. 6 × Juba Populus, and I-69 × Juba Populus. Improve the seedling rate of hybrid breeding offspring, specifically through the following:

[0034] 1) Use the pollen of Juba Yang to pollinate the pistils of Zhongqian No. 3, Zhongzhu No. 6 and I-69 poplars, and the pollen of Zhonghan 17 to pollinate the pistils of Danhong Yang. After 30 days, use scissors to cut from the base of the fruit stalk. Lower the capsule, rinse the surface of the peel with tap water, drain the water, and put it into a sterile bottle. Soak and sterilize with 75% alcohol for 6 minutes, then rinse with sterile water three times, put the material into a petri dish after rinsing, and absorb the moisture on the surface of the material with sterile filter paper.

[0035] 2) Gently press with a blade along the longitudinal a...

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Abstract

The invention discloses a method for increasing the survival rate of hybrid offspring seedlings of black poplar. The method comprises the following steps: 1) after pollination for 30-40 days, cutting capsules from the base parts of fruit stems, cleaning and disinfecting the capsules, and absorbing moisture on the surfaces of the capsules by using sterile filter paper; 2) peeling pericarp in the longitudinal axis direction of the pericarp of the capsules, and peeling flocculus together with embryos; (3) inoculating a 1 / 2MS culture medium with the flocculus and the embryos for culture until the seedling height is 4-6 cm or above and 4-6 true leaves exist; and 4) washing off the culture medium from tissue culture seedlings, transplanting the tissue culture seedlings into a non-woven fabric container filled with a substrate, and transplanting the tissue culture seedlings into a field after three weeks. The method is simple, feasible, safe and reliable, and the survival rate of the seedlings transplanted to the field reaches 95% or above.

Description

technical field [0001] The invention belongs to the technical field of plant seedling raising, and in particular relates to a method for improving the survival rate of black poplar hybrid offspring seedling raising. Background technique [0002] Plant tissue culture, also known as in vitro culture, uses isolated plant organs (such as roots, stems, leaves, stem tips, flowers, fruits, etc.), tissues or cells, and protoplasts in sterile and suitable artificial media and Under artificial conditions such as temperature, it can induce callus, adventitious buds, adventitious roots, and finally form a complete plant. Hybrid breeding is the main means of cultivating new varieties of poplar. In order to improve the breeding efficiency and solve the problems of ear shedding and immature seed development in the late stage of indoor cross-cutting and hydroponic hybridization, it is a feasible and effective method to culture hybrid embryos in the late stage of hydroponic culture. The ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/029A01G17/00A01G24/10A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01H4/008A01H4/001A01G31/00A01G24/28A01G24/15A01G24/10A01G17/005A01G9/0291A01G24/12Y02P60/21
Inventor 丁昌俊董玉峰张伟溪苏晓华李善文王延平黄秦军王雷
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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