Dopamine functionalized poly(beta-amino ester) as well as preparation method and application thereof

A dopamine and functionalized technology, applied in the biological field, can solve the problems of insufficient intracellular release and increased cytotoxicity, and achieve the effects of improving stability, low cytotoxicity and high transfection efficiency.
CN112876677AActive Publication Date: 2021-06-01ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
Publication Date
2021-06-01

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Abstract

The invention relates to the technical field of biology, and discloses dopamine functionalized poly(beta-amino ester) as well as a preparation method and application thereof. The structural formula of the poly(beta-amino ester) is shown in the specification, and the preparation method of the poly(beta-amino ester) comprises the steps: carrying out a polymerization reaction on 1,4-butanediol diacrylate and an amino-containing monomer; carrying out end capping on the polymer by adopting small molecular amine; and finally, carrying out deprotection on the phenolic hydroxyl group of dopamine to obtain the dopamine functionalized poly(beta-amino ester). Dopamine is introduced into PBAE, and ferric ions are complexed with the PBAE to form an encapsulated nanoparticle structure, so that the stability of a compound is improved, and a gene transfection vector with high transfection efficiency and low cytotoxicity is obtained, so that the gene transfection vector has potential clinical application value in the aspect of non-viral gene vectors.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a dopamine functionalized poly(β-amino ester) and its preparation method and application. Background technique

[0002] Interfering RNA (RNAi) is a naturally occurring cellular mechanism that culminates in the knockdown of specific genes. Targeted gene knockdown delivered by siRNA has potential clinical utility in treating diseases caused by abnormal gene expression. However, safe and efficient intracellular siRNA delivery remains challenging. Current studies have used the carrier materials (liposomes, inorganic materials and polymers) that can deliver DNA to deliver siRNA. Two major delivery hurdles unique to siRNA are: nanoparticle stability and cytoplasmic targeting. Since siRNA is approximately 200 times smaller than DNA plasmids and relatively rigid, it has low electrostatic interactions with cationic polymers, hindering the self-assembly of the two to form stable and small-s...

Claims

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