Bacillus coagulans with high lactic acid yield, biological fermentation feed as well as preparation method and application of biological fermentation feed

A technology of Bacillus coagulans and biologically fermented feed, applied in biochemical equipment and methods, methods based on microorganisms, animal feed, etc., can solve problems such as inability to meet the nutritional needs of different animals, limited nutritional components, poor quality or taste, etc. , to achieve the effects of improving palatability and production performance, improving lactic acid production performance, and improving intestinal health

Active Publication Date: 2021-06-15
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fermented soybean meal and other fermented products currently on the market have limited nu

Method used

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  • Bacillus coagulans with high lactic acid yield, biological fermentation feed as well as preparation method and application of biological fermentation feed
  • Bacillus coagulans with high lactic acid yield, biological fermentation feed as well as preparation method and application of biological fermentation feed
  • Bacillus coagulans with high lactic acid yield, biological fermentation feed as well as preparation method and application of biological fermentation feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation and Identification of Example 1 Bacterial Strain C12

[0033] 1. Isolation of Strain C12

[0034] Take 10g of a farm soil sample and place it in 100mL NJ liquid medium (glucose 0.5-1%, yeast extract 0.5-2%, peptone 1-2%, manganese sulfate 0.001-0.01%, dipotassium hydrogen phosphate 0.1-0.5%) , magnesium sulfate 0.05-0.5%, pH 5.5-7.0; the same below), enrichment culture at 160°C and 45°C for 24h. Select the bacterial solution at 80°C for 10min, spread on the plate, and incubate at 45°C for 24h.

[0035] 2. Purification of Strains

[0036] Pick a single colony for staining, microscopic examination, streaking and isolation of Gram-positive and rod-shaped bacteria, and save them.

[0037] 3. Screening of strains

[0038] Inoculate the single colony described in step 2 in NJ liquid medium, and culture at 45°C for 48 hours. As a result, a strain C12 with good acid production was obtained, and its characteristics were as follows: the microscopic examination show...

Embodiment 2

[0045] The preparation of embodiment 2 bacterial agents

[0046] The Bacillus coagulans of the present invention requires conditions for its liquid fermentation culture: glucose 0.5-1%, yeast extract 0.5-2%, peptone 1-2%, manganese sulfate 0.001-0.01%, dipotassium hydrogen phosphate 0.1-0.5 %, magnesium sulfate 0.05-0.5%, pH5.5-7.0;

[0047] After the above medium was inoculated, culture at 40°C for 2 hours statically;

[0048] The fermentation broth was centrifuged at 6000rad / min for 20min, and the bacteria were harvested;

[0049]Mix the bacteria with a 10% protective agent solution to prepare a suspension; the protective agent is selected from two or more of whey powder, skimmed milk powder, trehalose, sucrose, mannan oligosaccharides, etc.;

[0050] The above suspension is spray-dried, the air inlet temperature is 120°C, and the air outlet temperature is 70°C, to prepare a microbial agent with a biomass of up to 10 10 cfu / g.

Embodiment 3

[0051] The preparation of embodiment 3 biological fermentation feed

[0052] The present embodiment provides a kind of biological fermented feed, and its preparation method is as follows:

[0053] (1) Rejuvenation and multiplication of production bacteria

[0054] 1) Rejuvenation and propagation of Bacillus subtilis

[0055] Pick a ring of slant strains and inoculate them in the basic liquid medium. The medium capacity in a 250ml Erlenmeyer flask is 50ml. Cultivate in a shaker flask at 30-45°C and 160-220rad / min for 20-24h. This is the first-grade seed solution. The seed liquid is inserted into the secondary seed liquid culture medium with an inoculum amount of 2%, and the volume of liquid in a 1000ml Erlenmeyer flask is 400ml, and cultured in a shaker flask at 30-45°C and 160-220r / min for 20-24h; among them, the composition of the basic liquid culture medium It is: peptone 10-15g / L, yeast extract powder 5-10g / L, sodium chloride 5g / L, adjust the pH to 7.0-7.2; the compositio...

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Abstract

The invention provides bacillus coagulans with high lactic acid yield, biological fermentation feed as well as a preparation method and application of the biological fermentation feed. The preservation number of the bacillus coagulans is CGMCC No.21669. The biological fermentation feed preferably comprises bacillus subtilis, saccharomyces cerevisiae and a fermentation product of the bacillus coagulans. The biological fermentation feed provided by the invention contains rich small peptides, acidic substances, dietary fibers and other active ingredients, can emphatically improve the intestinal health of animals, improve the palatability and production performance of livestock, poultry and aquatic animals, reduce animal diarrhea, improve the product quality, kill enteropathogenic bacteria and reduce environmental pollution, has the advantages of being safe, non-toxic and free of residues and can replace antibiotics to a certain extent, and the preparation method is simple and feasible.

Description

technical field [0001] The invention relates to the technical field of microbes and functional feeds, in particular to bacillus coagulans with high lactic acid production, biological fermented feeds and their preparation methods and applications. Background technique [0002] Bacillus coagulans is a Gram-positive bacterium. Lactic acid production is an important property of Bacillus coagulans. Lactic acid is currently widely used in the field of feed additives. But the lactic acid production content of Bacillus coagulans mentioned in the reported literature is low at present, for example the lactic acid production amount of G4 bacterial strain is only 48.05mmol / L (4.32g / L) (Chen Xiuxiu etc., the isolation identification of a strain Bacillus coagulans and probiotic analysis [J], 2020, 11: 1-5). [0003] Fermented feed uses microorganisms and compound enzymes as biological feed starter strains to convert feed materials into microbial cell protein, biologically active small ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/56A23K10/12A23K10/30A23K10/37A23K20/20A23K50/75A23K50/80C12R1/07
CPCC12N1/20C12P7/56A23K10/12A23K10/30A23K10/37A23K20/20A23K50/75A23K50/80Y02P60/87Y02A40/818
Inventor 韩冰刘向阳肖进真诚贾红敏薛凌峰谭薇朱海源
Owner CHINA ANIMAL HUSBANDRY IND
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