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Preparation method for probiotics system and application of probiotics system

A probiotics and system technology, applied in the directions of non-active ingredients medical preparations, active ingredients-containing medical preparations, pharmaceutical formulas, etc., can solve the problems of complex operation, inability to form aggregation and self-protection system, not a treatment plan, etc. , to achieve a broad therapeutic effect, a broad tumor growth inhibitory effect, and the effect of improving the effect

Active Publication Date: 2021-06-18
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fecal microbial transplantation is costly, complicated to operate, and has a high risk of pathogen infection, so it is not a preferred treatment option
At the same time, various current practices and attempts have shown that direct oral administration of probiotics has little effect, and taking probiotics can only temporarily change the composition of intestinal microbiota in a short period of time, which is too short for these probiotics to exert their efficacy
The reason for this phenomenon is that the exogenous bacteria have poor habitat ability in the host intestine and cannot form a stable aggregation and self-protection system, so they are very easy to be directly eliminated by the gastrointestinal environment

Method used

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  • Preparation method for probiotics system and application of probiotics system
  • Preparation method for probiotics system and application of probiotics system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Experiments on melanoma mice

[0067] B16-F10 cells were obtained from the Cell Resource Center of Shanghai Academy of Biological Sciences, Chinese Academy of Sciences; SPF-grade C57BL / 6J mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.

[0068] The above-mentioned experimental objects were injected into the stomach of C57BL / 6J mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the B16-F10 tumor cells were counted by the red blood cell counting board, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 20 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical vertebrae, and the tu...

Embodiment 2

[0070] Embodiment 2: Breast cancer mouse experiment

[0071] 4T1 cells were obtained from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences; SPF-grade BALB / c mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.

[0072] The above-mentioned experimental objects were injected into the stomach of BALB / c mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the 4T1 tumor cells were counted by the red blood cell counting board, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 25 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical vertebrae, and the tumors w...

Embodiment 3

[0074] Embodiment 3: colon cancer mouse experiment

[0075] MC38 cells were obtained from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences; SPF-grade C57BL / 6J mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.

[0076] The above-mentioned experimental objects were injected into the stomach of C57BL / 6J mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the MC38 tumor cells were counted by the red blood cell counting board, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 25 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical vertebrae, and the tu...

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Abstract

The invention provides a preparation method for a probiotics system. The stable exogenous probiotics system can be obtained by using the method. After being taken, the probiotics system can form a stable microenvironment in a host, can take the effect of changing composition of intestinal microorganisms and finally exerts a positive role during treatment of a malignant tumor.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a probiotic system, in particular to a preparation method of a probiotic system which can be used for treating malignant tumors. Background technique [0002] Probiotics are a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. There are mainly lactic acid bacteria, bifidobacteria, actinomycetes, yeast and so on. They promote nutrient absorption and maintain intestinal health by regulating the immune function of the host mucosa and system or by regulating the balance of intestinal flora. Probiotics are generally considered to have multiple benefits to the human body, including: improving the body's immunity, maintaining the balance of the intestinal flora structure, improving the body's antioxidant level, inhibiting intestinal inflammation, and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K35/745A61K35/742A61K35/741A61K9/50A61K47/46A61P35/00A61K33/06A61K33/00A61K33/12
CPCA61K35/747A61K35/745A61K35/742A61K35/741A61K33/06A61K33/00A61K33/12A61K9/5063A61P35/00Y02A50/30A61K45/06A61K2300/00A61P1/16A61P17/00A61P1/12A61P11/00A61P1/04A61K35/744A61K47/02
Inventor 董磊韩丛薇
Owner NANJING UNIV
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