Probiotics system and application thereof
A technology of probiotics and system, applied in the field of probiotics, can solve problems such as complicated operation, inability to form aggregation and self-protection system, not a treatment plan, etc., and achieve extensive therapeutic effects, extensive tumor growth inhibition effects, and improved effects
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Embodiment 1
[0060] Example 1: Experiments on melanoma mice
[0061] B16-F10 cells were obtained from the Cell Resource Center of Shanghai Academy of Biological Sciences, Chinese Academy of Sciences; SPF-grade C57BL / 6J mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.
[0062] The above-mentioned experimental objects were injected into the stomach of C57BL / 6J mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the B16-F10 tumor cells were counted by the red blood cell counting plate, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 20 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical spine, the tumor was ...
Embodiment 2
[0065] Embodiment 2: Breast cancer mouse experiment
[0066] 4T1 cells were obtained from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences; SPF-grade BALB / c mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.
[0067] The above-mentioned experimental objects were injected into the stomach of BALB / c mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the 4T1 tumor cells were counted by the red blood cell counting board, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 25 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical vertebrae, and the tumors ...
Embodiment 3
[0070] Embodiment 3: colon cancer mouse experiment
[0071] MC38 cells were obtained from the Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences; SPF-grade C57BL / 6J mice weighing 18-20 grams were purchased from the Animal Experiment Center of Yangzhou University.
[0072] The above-mentioned experimental objects were injected into the stomach of C57BL / 6J mice by intragastric administration, and the dosage was calculated according to the amount of 0.01 g of mineral material for each mouse, once every two days, for a total of two weeks. On the fifteenth day, the MC38 tumor cells were counted by the red blood cell counting board, and then the cells were adjusted to 5×10 6 / ml, inoculated subcutaneously in the axilla of the right forelimb of the mouse (0.1ml / mouse) to establish a tumor model. 25 days after tumor implantation and 24 hours after the last administration, they were killed by dislocation of the cervical vertebrae, and the tu...
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