T cell serum-free culture medium and application thereof

Abstract

The invention relates to a T cell serum-free culture medium and an application thereof. The T cell serum-free culture medium is prepared by mixing a basic culture medium and additive ingredients, and the additive ingredients are composed of recombinant human insulin, recombinant human transferrin, recombinant human serum albumin, thymine, taurine and folinic acid; and the basic culture medium is one or a mixture of more of IMDM, DMEM, DMEM / F12, aMEM, RPMI1640 and M199. The culture medium provided by the invention not only has definite chemical components and no serum, human-derived and animal-derived components, but also has fewer types of added components, a simplified formula and excellent performance, the T cells can be amplified by 400-1000 times after being cultured for 10-12 days, and the end cell viability can reach 85-95%.

Description

Technical field [0001] The present invention relates to the field of cell culture techniques, and in particular, there is a T cell-free-serum-free medium and its applications thereof. Background technique [0002] T cells are the main components of lymphocytes, which have a variety of biological functions, such as direct killing target cells, assisted other lymphocytes to function, respond to specific antigen or migratic original responses, and producing cytokines, Body resists disease infection to prevent one of the major immune cells formed by tumors. In vitro, activated, amplified such immune cells, can be used to return to the treatment of a variety of diseases, including malignant tumors, infection, autoimmune diseases. During this process, the use of safe and reliable, chemical components, explicit medium, to avoid the possible human source, animal source pathogens, while avoiding the immune immunity that the T cell amplification system summarizes unknown ingredients may ca...

AI Technical Summary

Problems solved by technology

The problem of this medium is: 1) the difference between serum batches leads to unstable expansion of T cells; 2) the serum components containing xenogeneic animal (generally bovine) sources, there is a risk of introducing xenogeneic animal pathogen infection, which increases the The risk of clinical use; 3) The composition of serum is complex, which contains a variety of proteins, growth factors, etc., some of which can lead to excessive activation and exhaustion of T cells, and cannot achieve the best T cell expansion effect; 4) In the culture system Possible residues of xenogeneic animal-derived components increase the difficulty of quality control and clinical declaration of cell therapy products

[0004] The existing commercial T cell serum-free medium has the following problems: 1) The composition of the formula is complicated, and at least a dozen other components need to be added in addition to the basic medium; 2) Most of them need to add human albumin derived from human serum , which increases the complexity of the ingredients, the instability of the source of raw materials and the difficulty of manufacturing, the real chemical composition cannot be achieved, and the clinical use has great uncertainty

However, there are still many defects in the addition of components, the amplification efficiency is slightly insufficient, and the cell quality needs to be improved

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