A rapid thin-layer identification method for Qiling granules with one test and multiple drugs
A detection method and particle technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of the detection personnel's health and adverse environmental effects, and achieve the effects of saving solvent and labor costs, simple operation, and quality control
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Embodiment 1
[0043] TLC identification of Qiling Granules:
[0044] (1) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain the product.
[0045] (2) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixture of salvianolic acid B, gentiopicroside each 1 mg / mL, and astilbin 1.5 mg / mL Reference substance solution, that is, too.
[0046] (3) Preparation of negative reference substance solution: Take 0.5 g of each negative particle, add 5 mL of methanol, sonicate for 30 min, and take the supernatant to obtain the product.
[0047] (4) Draw 4 μL of the test solution, 4 μL of the reference solution, and 4 μL of the negative control solution, and spot on the same silica gel GF 254 On a thin-layer board, use petroleum ether-ethyl acetate-methanol-anhydrous formic acid (2:7:1:0.5) as a developing agent, develop the filter paper in the deve...
Embodiment 2
[0050] The present embodiment investigates with different spotting methods and sample volumes of the test solution, to further illustrate the technical solution.
[0051] (1) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain the product.
[0052] (2) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixed control of salvianolic acid B, gentiopicroside 1mg / mL, and astilbin 1.5mg / mL Product solution, that is.
[0053] (3) Preparation of the negative control solution: Take 0.5 g of each negative particle, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain.
[0054] (4) Take 2 μL, 4 μL, 8 μL, and 10 μL of the different batches of the test solution, 4 μL of the reference solution, and 4 μL of the negative control solution, and spot on the same silica gel GF 254 On a thin-layer board, use p...
Embodiment 3
[0057] In this embodiment, the different sample volumes of the reference substance solution and the negative control solution are investigated to further illustrate the technical solution.
[0058] (1) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixed control of salvianolic acid B, gentiopicroside 1mg / mL, and astilbin 1.5mg / mL Product solution, that is.
[0059] (2) Preparation of negative control solution: take 0.5 g of each negative particle, add 5 mL of methanol, ultrasonicate for 10 min, and take the supernatant to obtain the product.
[0060] (3) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 10 min, and take the supernatant to obtain the product.
[0061] (4) Draw 1 μL, 3 μL, 5 μL of the reference substance solution, 2 μL, 4 μL, 8 μL, 10 μL of the negative control solution, and spot on the silica gel GF 254 On a thin-layer board, use petroleum...
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