A Method for Visual Detection of Substance Concentration Based on Mobile Exchange Interface

Abstract

A method for visually detecting substance concentration based on a mobile exchange interface, which mainly includes: 1) different ligands undergo displacement reactions on the surface of solid-phase substances (such as nanoparticles, cells, etc.) to form optical signals; The gel (or self-filling, etc.) is fixed in the electrophoresis channel, and the analyte is driven to move in the channel by electrophoresis and replaces the ligand on the surface of the solid-phase substance to form a mobile exchange interface; 3) Record the interface movement to establish the interface migration The standard curve of rate, interface width, and species concentration is used to calculate the species concentration (or ligand displacement reaction rate) that reacts on the surface of the solid phase species. The method has the advantages of simple operation process, low cost, visualization and no need to rely on expensive instruments.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, nanoscience, biotechnology and the like, in particular to a method for visually detecting substance concentration based on a mobile exchange interface. Background technique [0002] After years of theoretical and applied research, the moving interface theory has been proven to have the potential to detect trace substances. The related work has applied for a patent on moving interface detection (electromigration acid-base titration device for determining acid-base concentration, China, 2010 -12-18, ZL201010596012.7; A visual biosensor device for measuring total protein concentration, China, 2012-05-09, ZL201210142985.2; High-throughput protein titration method based on mobile reaction interface electrophoresis, China, 2013-03-20, ZL201310089084.6; anti-leakage electrophoresis titration electrode solution flow exhaust device and its use method, China, 2014-09-18, ZL201410478864.4, etc.). [00...

AI Technical Summary

Problems solved by technology

[0004] Although the mobile reaction interface has been used in the detection of protein and nucleic acid content, and can successfully measure the ligand content on the surface of quantum dots, the mobile interface formed by ligand exchange on the surface of the material has not yet been studied in depth, especially The research and application of the ligand exchange reaction based on electrophoresis technology to detect the concentration of the analyte is relatively lacking

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