Device and method for measuring active oxygen content in sperm

Abstract

The invention discloses a sperm active oxygen content measuring device, which comprises a base, a mounting plate fixed on the base, a swing plate hinged on the mounting plate, a first test tube fixed on the swing plate, the lower end of the first test tube It is set through the swinging plate, and its bottom is connected with a valve I, the bottom of the valve I is connected with a second test tube, the bottom of the second test tube is connected with a throttling valve, and both the first test tube and the second test tube are fixed with ultrasonic generators , the mounting plate is also provided with a swinging plate drive mechanism located below the swinging plate, the swinging plate driving mechanism includes a fixed plate, a motor A, a reducer A and a cam, the fixed plate is fixed on the mounting plate, and is located directly below the swinging plate . The invention has the beneficial effects of compact structure, improved detection efficiency of active oxygen content in semen, high mixing efficiency, uniform mixing and simple operation.

Description

technical field [0001] The invention relates to the technical field of in vitro detection of sperm biochemical indicators, in particular to a sperm reactive oxygen species content determination device and a determination method thereof. Background technique [0002] Reactive oxygen species (ROS) refers to a class of oxygen-containing substances with active chemical properties and strong oxidative ability in the body or in the natural environment. Reactive oxygen species are metabolites of oxygen, including superoxide anion, hydrogen peroxide, hydroxyl group, peroxy hydroxyl radical, nitrous oxide, etc. Excessive concentrations of reactive oxygen species can induce oxidative damage to lipids, proteins and DNA in cells, thereby initiating pathological changes. [0003] The female infertility caused by the male is called male infertility, which accounts for about 50% of the entire infertility factor, and high levels of reactive oxygen species are found in 25%-40% of the infert...

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Problems solved by technology

[0005] Yet, though this method can measure sperm active oxygen content, still there is following defect: 1, when artificial shake test tube, need to shake for a long time and could thoroughly shake the semen to be detected and the phosphate buffered saline solution thoroughly, prolonged the semen Detection time, thereby reducing the detection efficiency of reactive oxygen species in semen

II. When manually shaking the test tube, the mixture of semen and phosphate buffer solution may be shaken out, which is very inconvenient to clean the table in the later stage

III. After mixing twice, it is necessary to drop the mixed solution of semen and DCFH-DA fluorescent probe solution onto the smear, and then place the smear under a fluorescent microscope for observation, which undoubtedly increases the measurement process and further Reduced the detection efficiency of active oxygen content in semen

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