Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof

A technology of thyroglobulin and colloidal gold test paper, which is applied in the field of immunoassay, can solve the problems that the Tg detection method is not suitable for on-site rapid detection, and achieve the effect of fast detection speed, strong specificity and easy use

Pending Publication Date: 2021-07-09
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, in order to solve the problem that the existing Tg detection method is not suitable for on-site rapid detection, a proposed immunocolloidal gold test strip for detecting thyroglobulin and its preparation method

Method used

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  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof
  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof
  • Immune colloidal gold test strip for detecting thyroglobulin and preparation method thereof

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] 1. Main material:

[0027] Human thyroglobulin full length protein (Tg freeze-dried powder ab96518), Anti-Thyroglobulin antibody (5F9) (anti-thyroglobulin antibody, ab1984), Anti-Thyroglobulin antibody (5E6) (anti-thyroglobulin antibody, ab1982): abcam company;

[0028] Goat anti-mouse IgG, colloidal gold test strip experimental consumables package: Shanghai Jinbiao Biotechnology Co., Ltd.;

[0029] BSA: Beijing Suolaibao Biotechnology Co., Ltd.;

[0030] Sodium citrate, chloroauric acid: Tianjin Yingda Rare Chemical Reagent Factory;

[0031] Clinical samples: 3 in total, 2 serum samples (Tg value 1541ng / ml, 20ng / ml), 1 cervical lymph node puncture eluate sample (Tg value 46ng / ml when diluted 1000 times).

[0032] 2. Preparation method:

[0033] (1) Preparation of colloidal gold: add 99ml of ultrapure water to the flask, heat to boiling state...

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Abstract

The invention discloses application of an immune colloidal gold test strip for detecting thyroglobulin in preparation of a tool for rapidly detecting a thyroglobulin value of a suspicious lymph node on site in an operation and relates to the technical field of immunoassay. In the application, the immune colloidal gold test strip comprises a sample pad, a gold label pad, a nitrocellulose film and a water absorption pad which are partially spliced on a PVC (polyvinyl chloride) bottom plate in sequence, the gold label pad is coated with a colloidal gold labeled anti-thyroglobulin antibody, and a detection line and a quality control line which are separated are arranged on the nitrocellulose film. The detection line is coated with another anti-thyroglobulin antibody, and the quality control line is coated with goat anti-mouse IgG. The invention further provides application of the immune colloidal gold test strip in preparation of a tool for auxiliary diagnosis of lymph node metastasis of papillary thyroid carcinoma and application of the immune colloidal gold test strip in preparation of a tool for detecting thyroglobulin value in lymph node fine needle puncture suction eluent.

Description

[0001] This application is a divisional application of an invention patent application with an application date of 2017.04.28, an application number of 201710290582.5, and an application name of "Immune colloidal gold test strip for detecting thyroglobulin and its preparation method". The applicant is the general manager of Tianjin Medical University. Hospital. technical field [0002] The invention relates to an immunoassay method, in particular to an immune colloidal gold test strip for detecting thyroglobulin and a preparation method thereof. Background technique [0003] Thyroglobulin (Tg) is a glycoprotein with a molecular weight of about 660,000 secreted by the thyroid follicular epithelium, and it is one of the basic raw materials for the synthesis of thyroid hormones. Determination of blood Tg level and lymph node fine needle aspiration eluate Tg level (FNA-Tg) are widely used in the diagnosis, treatment and follow-up of differentiated thyroid cancer, especially papi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544
CPCG01N33/544G01N33/558
Inventor 何向辉王义增
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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