Tissue culture and rapid propagation method for virus-free high-quality seedlings of canna generalis

A technology of canna large flower and tissue culture, applied in the field of plant propagation, to achieve the effect of high application value and rapid reproduction

Active Publication Date: 2021-07-13
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the method of rapid propagation of canna grandiflora by tissue culture of high-quality seedlings free of virus at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Obtaining and disinfection methods of explants:

[0022] Select a good mother plant with strong growth and no symptoms of virus infection on the surface, dig out the 2-4 cm long shoots on the rhizome, wipe it with a damp cloth, then wipe the surface with 75% ethanol cotton by volume fraction, soak in 75% by volume fraction % alcohol for 10 seconds, then sterilized with 1% sodium hypochlorite solution for 5 minutes, rinsed with sterile water for 4 times, peeled off the young leaves, and then sterilized with 0.1% mercuric solution for 2 minutes. After washing with sterile water for 4 times, the outer young leaves were peeled off, and then disinfected with 0.1% mercury liter solution for 1 minute. After 4 times of sterile water washing, 2 mm shoot tips were stripped for primary culture. The primary medium is MS medium + 3.0 mg / L 6-benzylpurine (6-BA) + 12 mg / L antiviral ether + 20 g / L sucrose + 6 g / L agar, pH 5.8, first in dark conditions Under culture 5 days (if do no...

Embodiment 2

[0032] 1. Obtaining and disinfection methods of explants:

[0033]Select a good mother plant with strong growth and no symptoms of virus infection on the surface, dig out the 2-4 cm long shoots on the rhizome, wipe it with a damp cloth, then wipe the surface with 75% ethanol cotton by volume fraction, soak in 75% by volume fraction % alcohol for 20 seconds, then sterilized with a mass fraction of 1% sodium hypochlorite solution for 6 minutes, rinsed with sterile water for 4 times, peeled off the outer tender leaves, and then put in a mass fraction of 0.1% mercury liter solution for 3 minutes, no After washing with sterile water for 5 times, the young leaves outside were peeled off, and then put into 0.1% mercuric chloride solution for disinfection for 2 minutes. After washing with sterile water for 5 times, 3 mm shoot tips were stripped for primary culture. The primary medium is MS medium + 4.0 mg / L 6-benzylpurine (6-BA) + 10 mg / L antiviral ether + 25 g / L sucrose + 6 g / L agar,...

Embodiment 3

[0043] 1. Obtaining and disinfection methods of explants:

[0044] Select a good mother plant with strong growth and no symptoms of virus infection on the surface, dig out the 2-4 cm long shoots on the rhizome, wipe it with a damp cloth, then wipe the surface with 75% ethanol cotton by volume fraction, soak in 75% by volume fraction % alcohol for 30 seconds, then sterilized with a mass fraction of 1% sodium hypochlorite solution for 7 minutes, rinsed with sterile water for 6 times, peeled off the outer tender leaves, and then sterilized with a mass fraction of 0.1% mercury liter solution for 4 minutes. After 6 times of bactericidal water washing, the outer young leaves were peeled off, and then disinfected with 0.1% mercury liter solution for 3 minutes. After 6 times of sterilized water washing, 4 mm stem tips were stripped for primary culture. The primary medium is: MS medium + 5.0 mg / L 6-benzylpurine (6-BA) + 8 mg / L antiviral ether + 30 g / L sucrose + 6 g / L agar, pH 6.0, firs...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method for virus-free high-quality seedlings of canna generalis. The tissue culture and rapid propagation method comprises the following steps that a, explants are obtained and disinfected; b, adventitious buds are induced and subculture proliferation is carried out; c, rooting culture is carried out; d, test-tube plantlets are transplanted; and e, virus detection is carried out. The high-quality virus-free seedlings are produced by taking stem tips as the explants and combining high-temperature and antiviral agents to remove viruses, rapid propagation of the canna generalis is realized, and the application value is high. The tissue culture and rapid propagation method can be implemented only by simple plant tissue culture equipment.

Description

technical field [0001] The invention belongs to the field of plant propagation, and in particular relates to a rapid propagation method for virus-free high-quality seedling tissue culture of Canna grandis. Background technique [0002] Canna x generalis is a horticultural hybrid belonging to the genus Canna of the family Cannaceae, native to tropical America and Africa. It has lush branches and leaves, gorgeous flowers, graceful posture, and long flowering period. It can bloom almost all year round in southern China. It is an excellent variety for large flower beds. However, canna large flowers are often susceptible to virus infection during the growth process, especially when rhizomes are often used for branch propagation, and the virus will accumulate and be passed on to offspring. Cannas infected with viruses mainly show symptoms such as mosaic leaves, chlorotic stripes, small flowers with variegated spots, and plant dwarfing, which affect the normal growth and ornamenta...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君吴坤林李琳房林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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