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Pseudomonas aeruginosa and application thereof

A technology of Pseudomonas aeruginosa and pathogenic bacteria, applied in the field of microorganisms, can solve problems such as polluting the environment, pesticide residues, and affecting the quality of medicinal materials, and achieve broad application prospects, high-efficiency antagonism, and good growth-promoting properties.

Active Publication Date: 2021-07-23
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]At present, the disease control of saffron mainly depends on chemical fertilizers and pesticides. Although it has the advantages of rapid onset and long-lasting effect, it will cause pesticide residues and pollute the environment, etc. problems, not only will affect the quality of medicinal materials, but also inconsistent with the national strategy of green development and ecological agriculture

Method used

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  • Pseudomonas aeruginosa and application thereof
  • Pseudomonas aeruginosa and application thereof
  • Pseudomonas aeruginosa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Isolation and screening of YY322 bacterial strain

[0022] 1) Collection of samples

[0023] Collect healthy saffron rhizosphere soil from Shanghai Chongming Island (121°09′30″~121°54′00″ east longitude, 31°27′00″~31°5l′15″ north latitude) and seal it in a sterile ziplock bag , transported back to the laboratory in an ice box, and dried in the shade for two weeks in a clean and ventilated place for later use.

[0024] 2) Isolation and purification of bacteria

[0025] After crushing the naturally dried soil with a mortar, pass through a 20-mesh sieve, weigh 5g of the soil sample and place it in a triangular flask filled with 45mL of sterile water, add a little glass beads to the triangular flask, and place it at 150rmp Oscillate on a shaker for 2 hours and stand still for 1 hour, then draw 1 mL from it and place it in a test tube filled with 9 mL of sterile water, shake fully, and make 10 -2 soil dilution, and so on, to make 10 -3 、10 -4 、10 -5 The s...

Embodiment 2

[0034] Embodiment 2: Identification of YY322 bacterial strain

[0035] 1) Physiological and biochemical identification

[0036] With reference to "Common Bacteria System Identification Handbook" and "Bergey's Bacteria Identification Handbook", the YY322 bacterial strain obtained by screening in Example 1 was inoculated on LB medium and cultivated for 24 hours, and then observed the growth situation of antagonistic bacteria, colony shape and color, and bacterial strains. Ram's staining and other morphological and physiological and biochemical characteristics.

[0037] figure 2 is the morphological feature map of the YY322 bacterial strain, composed of figure 2 It can be seen that the strain grows rapidly, and the single colony is nearly round, with irregular edges, relatively flat, light yellow-green, not smooth, and opaque.

[0038] The specific physiological and biochemical identification results are shown in Table 1:

[0039] Table 1 Physiological and biochemical chara...

Embodiment 3

[0052] Embodiment 3: detection YY322 bacterial strain produces the performance of degradative enzyme

[0053] 3.1. Protease

[0054] Pick the activated YY322 strain with a sterile toothpick and spot it on the protease detection plate (15g of skim milk powder, 18g of agar, 1L of distilled water, sterilized at 115°C for 15min), repeat 3 times, and culture in a 30°C incubator for 2 days Observe that a clear and transparent circle is positive.

[0055] 3.2. Chitinase

[0056] Pick the activated YY322 strain with a sterile toothpick and spot on the chitinase detection plate (NH 4 h 2 PO 4 1g, KCl0.2g, MgSO 4 ·7H 2 O 0.2g, colloidal chitin 10g, agar 18g, distilled water 1L, sterilized at 121°C for 20min), repeat 3 times, observe after 2 days in a 30°C incubator, and a clear transparent circle is positive.

[0057] 3.3 Cellulase

[0058] Pick the activated YY322 strain with a sterile toothpick and spot on the cellulase detection plate ((NH 4 ) 2 SO 4 2g, MgSO 4 ·7H 2 O...

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Abstract

The invention discloses Pseudomonas aeruginosa and application thereof. The classification name of the Pseudomonas aeruginosa is Pseudomonas aeruginosa YY322, the preservation number is CCTCC NO: M2021208, and the Pseudomonas aeruginosa is preserved in the China Center for Type Culture Collection on March 8, 2021. Research results show that the Pseudomonas aeruginosa and volatile gas generated by the Pseudomonas aeruginosa have an efficient antagonistic effect on stigma croci pathogenic bacteria and can be used for preventing and treating stigma croci diseases caused by the stigma croci pathogenic bacteria; in addition, experimental results also prove that the Pseudomonas aeruginosa has the effects of producing protease, producing glucanase, fixing nitrogen, dissolving phosphorus, producing siderophore and producing NH3 and shows good growth promoting performance; and therefore, the Pseudomonas aeruginosa is expected to be used for preparing a soil conditioner or / and fertilizer for preventing and treating the stigma croci diseases and has wide application prospects.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa and its application, in particular to a strain of Pseudomonas aeruginosa YY322 and its application in preventing and treating saffron pathogenic bacteria, and belongs to the technical field of microorganisms. Background technique [0002] Saffron (Crocus sativus) is a perennial flower of the genus Crocus in the family Iridaceae. It is a world-renowned dye and spice, and is also an important medicinal plant. The catalog of homologous substances in medicine and food has great development and utilization value. At the same time, because its medicinal parts are only dry stigmas, the yield is extremely low, so the market price is extremely high, and the high-quality ones can reach about 50 yuan per gram. It is known as "red gold", so the research on its cultivation and planting is particularly important. . [0003] Saffron is native to Iran, Greece, India, and Spain. It was introduced in my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P3/00C05F11/08C12R1/385
CPCA01N63/27C05F11/08
Inventor 胡双黎万奎王莉莉孙文静汪星星
Owner SHANGHAI UNIV OF T C M
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