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Methylotrophic bacillus strain and its application

A methylotrophic, bacillus technology, applied in the application, bacteria, fungicides and other directions, can solve the problem of single function of bacillus, and achieve good development and application prospects, high effect, and large biological control effect.

Active Publication Date: 2018-04-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functions of these bacillus are relatively simple, and the isolation and screening of high-efficiency strains with antagonistic effects on various plant pathogenic bacteria is the hot spot and key issue of current research.

Method used

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  • Methylotrophic bacillus strain and its application
  • Methylotrophic bacillus strain and its application
  • Methylotrophic bacillus strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The screening and identification of methylotrophic Bacillus strain CGMCC No.10779 includes the following steps:

[0028] (1) Activation of pathogenic bacteria

[0029] The inoculation needle uses aseptic operation to hook a small amount of anthracis anthracis hyphae and medium to be activated into the center of the PDA plate, inoculate 3 pieces respectively, and conduct constant temperature culture at 26°C to prepare a spore suspension;

[0030] (2) Sample collection and preparation of soil suspension

[0031] Collect bacterial samples from the soil of willow fields in Xuancheng area. When collecting soil samples, first remove the topsoil and take the soil sample at a depth of 5-20cm; use the dilution method to separate, weigh 10.0g soil sample and pour it into 100ml sterile water Shake well in the Erlenmeyer flask, then put it in a water bath at 80℃ to heat (shaking every 3min), put it in cold water to cool down after 20min, and then gradually dilute to prepare a soil suspensi...

Embodiment 2

[0053] Effect of Methylotrophic Bacillus strain CGMCC No. 10779 on the growth of Colletotrichum gloeosporioides, Colletotrichum gloeosporioides, Fusarium wilt of watermelon, Fusarium wilt of cotton and pathogen of corn leaf spot

[0054] 2.1 Determination of the inhibitory rate of methylotrophic Bacillus strain CGMCC No.10779 against Colletotrichum gloeosporioides, Colletotrichum gloeosporioides, Fusarium wilt of watermelon, Fusarium wilt of cotton, and pathogen of corn leaf spot

[0055] The plate confrontation method was used to detect the inhibitory effects of the methylotrophic Bacillus strain CGMCC No.10779 on Colletotrichum gloeosporioides, Colletotrichum gloeosporioides, Fusarium wilt of watermelon, Fusarium wilt of cotton and pathogen of corn leaf spot. The test method is: inoculate the plant pathogens on the PDA plate, culture at 26°C for 3 days, use a punch with a diameter of 5 mm to pick out the colonies with consistent growth, spot them in the center of the PDA plate, an...

Embodiment 3

[0066] Effect of methylotrophic Bacillus strain CGMCC No.10779 on the hyphae of Colletotrichum gloeosporioides and watermelon wilt pathogen

[0067] Inoculate anthracnose and watermelon fusarium wilt pathogens on the PDA plate. After incubating at 26°C for 3 days, use a punch with a diameter of 5 mm to pick out the colonies with uniform growth and spot them in the center of the PDA plate, 2 cm from the side of the pathogen Streak access to the methylotrophic Bacillus strain CGMCC No.10779, use only pathogenic bacteria as the control, 3 replicates for each treatment, culture at 26°C; wait until the pathogenic bacteria in the control group grows over the entire petri dish, observe its inhibition For the presence or absence of bacterial bands, observe its inhibitory effect. The mycelium was selected, fixed with glutaraldehyde, processed by conventional methods, and observed by scanning electron microscope. Put the selected mycelium block (5mm×5mm, thickness about 3mm) into 4% gluta...

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Abstract

The methylotrophic Bacillus strain has the preservation number: CGMCC No.10779. The present invention also provides the application of the methylotrophic bacillus strain CGMCC No. 10779 in antagonizing plant pathogenic bacteria, especially the application in antagonizing Ophiopogon japonicus anthracnose pathogenic bacteria. Beneficial effect of the present invention is reflected in: 1. methylotrophic bacillus strain CGMCC No.10779 of the present invention has antagonism to multiple plant pathogenic bacteria, such as watermelon wilt, cotton wilt, corn small spot pathogenic bacteria, especially to wheat The pathogenic bacteria of winter anthracnose has a very efficient antagonistic effect. It is an antagonistic strain with high effect on plant disease control, environmental friendliness and great biological control. It has a good development and application prospect. 2. The methylotrophic bacillus strain CGMCC No.10779 of the present invention has strong salt tolerance, can still grow on a medium with a sodium chloride content of 9%, has strong stress resistance, and can be applied to land with relatively harsh environments. Potential application value is high.

Description

Technical field [0001] The invention belongs to the field of development and application of microorganisms, and specifically relates to a methylotrophic Bacillus strain and its application. Background technique [0002] With the requirements of intensification and sustainable development of agricultural production, crop diseases and insect pests have become a bottleneck restricting the sustainable development of modern agriculture. In particular, soil-borne diseases such as fusarium wilt, verticillium wilt, root rot, sclerotinia, bacterial wilt, etc., are spread through the soil, causing the disease of crop roots and whole plants, and causing serious losses to agricultural production. However, the large amount of chemical pesticides used now cause pollution of soil and water resources, which seriously threaten people’s health. The breeding cycle of disease-resistant varieties is very long. Due to the limitations of the above traditional methods, it is necessary to find an environ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 杨恩东孙乐妮孙冬冬汪维云蔡永萍陈晓琳孙旭常艳
Owner ANHUI AGRICULTURAL UNIVERSITY
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