Biological control agent for fusarium wilt and preparation and use method of biological control agent

A biological control agent and fusarium wilt technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problem that the number of effective viable bacteria cannot be determined, the efficacy cannot be exerted, and the scope of use is limited and other problems, to achieve the effect of improving disease resistance and prevention effect, good synergistic effect of promoting growth, and small molecular weight

Active Publication Date: 2013-12-25
ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of the composite bacterial agent disclosed in this patent adopts mixed fermentation of multiple strains. Due to the large difference in the culture conditions of each strain, the effective number of viable cells of each strain in the final product cannot be determined, and it is impossible to make each strain If the efficacy of the product is brought into play, the use effect of the product will be limited; in addition, the compound bacterial agent is only for the control of cucumber wilt, and the scope of application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] According to the volume ratio, 10% of the cellulase-producing Trichoderma viridans suspension, 10% of the Aspergillus niger suspension, 8% of the Bacillus subtilis suspension, 10% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 62%, mix uniformly, obtain biological control agent.

[0024] Taking watermelon as the experimental object, a seed soaking and root irrigation test was carried out: the biological control agent was diluted 100 times with water, the watermelon seeds were soaked in the diluted solution of the biocontrol agent for 5 hours, and then planted. The biological control agent is diluted 100 times with water according to the consumption rate of 5 liters per mu, and is watered twice on the roots of the plants during the growing period of the watermelon and the period of vigorous fruit growth.

[0025] Effect: the incidence rate of watermelon wilt in the experimental group of the present invention is 5%, that in the chemical control group i...

Embodiment 2

[0027] According to the volume ratio, 15% of the cellulase-producing Trichoderma viridans suspension, 8% of the Aspergillus niger suspension, 10% of the Bacillus subtilis suspension, 12% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 55%, mix uniformly, obtain biological control agent.

[0028] Taking tomato as the experimental object, conducting seed soaking and spraying tests: adding water to dilute the biological control agent 200 times, soaking tomato seeds for 8 hours, and planting. During the period of tomato growth and expansion, the biological control agent is diluted 20 times with water at an amount of 1 liter per mu, and sprayed on the leaves, leaf backs, fruits and young stems; spray 2 times with an interval of 1 week.

[0029] Effect: the incidence rate of tomato fusarium wilt in the experimental group of the present invention is 8%, that in the chemical control group is 15%, and that in the control group without control is 22%; the tomato outpu...

Embodiment 3

[0031]According to the volume ratio, 20% of the cellulase-producing Trichoderma viridans suspension, 5% of the Aspergillus niger suspension, 15% of the Bacillus subtilis suspension, 8% of the colloid Bacillus suspension, biochemical fulvic acid Liquid 52%, mix uniformly, obtain biological control agent.

[0032] Taking cucumber as the experimental object, conducting seed soaking, root irrigation and spraying tests: adding water to dilute the biological control agent 150 times, soaking cucumber seeds for 10 hours, and planting. During the growth and colonization period of cucumbers, dilute the biological control agent 200 times with water at an amount of 2 liters per mu, and pour it on the roots of the plants; Dilute it 200 times and pour it on the roots of the plants. During the fruiting period of cucumbers, the biological control agent was diluted 40 times with water at a dosage of 2 liters per mu, and sprayed on the leaves, leaf backs, fruits and young stems; sprayed 3 time...

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Abstract

The invention discloses a biological control agent for fusarium wilt and a preparation and use method of the biological control agent. The biological control agent comprises the following components of not less than 1*10<7>cfu / mL of cellulose-producing trichoderma viride, not less than 0.5*10<7>cfu / mL of aspergillus niger, 0.8*10<9>cfu / mL of bacillus subtilis and 0.8*10<9>cfu / mL of bacillus mucilaginosus. The prepared biological control agent has an antagonism on antagonism, is capable of increasing disease prevention capability, and has functions of activating soil nutrients, promoting growth of root systems of crops, and inducing the improvement of disease resistance when being applied, thereby improving control effect of fusarium wilt of the crops, and enabling the incidence rate to be lower than 10 percent. The biological control agent can completely replace a chemical agent, has no pollution, residue and biological toxicity during use, ensures that the yield of crops is increased by above 15 percent, and is suitable for controlling fusarium wilt of cucurbit plants such as cucumber, watermelon, muskmelon, bottle gourd and towel gourd, and solanaceae crops such as tomato and eggplant.

Description

technical field [0001] The invention belongs to the technical field of fruit and vegetable disease prevention and control, and in particular relates to a biological control agent for fusarium wilt and its preparation and use method. Background technique [0002] Crop Fusarium Wilt (Cucurbits Fusarium Wilt), also known as vine cutting disease and wilting disease, is an important soil-borne disease of crops. It is widely distributed and can occur in both seedling and adult plant stages. It mainly harms cucumbers, watermelons, melons, Cucurbitaceous crops such as winter melon, and solanaceous crops such as tomato, eggplant, and potato. Fusarium wilt is caused by fungal infection of the genus Fusarium. After identification, there are two species in my country: Fusarium oxysorum Schlecht. and Fusarium bulbigenum Cke.et Mass.var.niveum (E.F.Sm) Wr.). The viability of the bacteria is very strong, and it can survive in the soil for 5-6 years. [0003] Fusarium wilt is a serious s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N63/00A01P3/00C09K17/00C09K101/00
Inventor 赵文娟徐升运马齐陈五岭任平秦涛张强
Owner ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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