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Single-core bird's beak-shaped optical fiber optical tweezers with sorting function and preparation method thereof

An optical fiber optical tweezers and bird's beak technology, applied in the field of light manipulation, can solve the problems of low sorting rate, cell damage, large limitations, etc., and achieve the effect of a wide range of applications

Active Publication Date: 2022-07-29
GUILIN UNIV OF ELECTRONIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the above-mentioned methods all have certain limitations, such as the extracellular vesicle surface protein-specific aptamer screening technology based on the immunomagnetic bead method (patent number: CN110791504A) and the detection reagent and its preparation method based on the immunomagnetic bead method, In the kit and detection method (Patent No.: CN111579772A), the immunomagnetic bead method is used to sort the target cells, and the sorting procedure and the preparation of the detection reagent are relatively complicated; another example is a sorting method of human osteoblasts ( Patent No.: CN111560347A) Centrifugation is used for cell sorting, which is to sort the quality of cells, and the sorting of particle size cannot be realized, which has relatively large limitations and low sorting rate; in addition, flow cytometry, fluorescence activated Although the laser induction method and the laser induction method can be used as effective means to separate cells, these three methods need to add fluorescent markers or other treatments to the cells, which changes the original state of the cells, sometimes affects the cell viability, and is easy to cause damage to the cells.

Method used

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  • Single-core bird's beak-shaped optical fiber optical tweezers with sorting function and preparation method thereof
  • Single-core bird's beak-shaped optical fiber optical tweezers with sorting function and preparation method thereof
  • Single-core bird's beak-shaped optical fiber optical tweezers with sorting function and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Cell sorting with a bird's beak-shaped fiber optic probe with a radius of curvature of 75 μm

[0032] In the system, a laser with a wavelength of 940 nm is passed into the single-mode fiber, and is adjusted by a three-dimensional adjustment frame during the transmission process. Displayed on a PC by a microscope imaging system. like Image 6 shown, when the radius of curvature of the bird’s beak-shaped fiber probe is 75 μm, due to Y b5 The evanescent field gradient force experienced by the particle at the location is less than the light scattering force, so cells cannot be trapped at that location. while in Y b1 ~Y b4 The gradient force on the positional particles is greater than the light scattering force, so cells can be trapped at these four positions. and Y b1 ~Y b3 The integral value of the stress tensor showed an increasing trend, and the integral value of the stress tensor was 1.59pN / m, 1.94pN / m and 2.60pN / m, respectively. while Y b4 The integr...

Embodiment 2

[0033] Example 2 Cell sorting with a bird's beak-shaped fiber optic probe with a radius of curvature of 100 μm

[0034] like Image 6 shown, when the radius of curvature of the bird’s beak fiber probe is 100 μm, Y b5 Place cells still cannot be captured. while in Y b1 ~Y b2 The stress tensor integral values ​​of place cells showed an increasing trend, which were 1.07pN / m and 2.84pN / m, respectively. while Y b3 ~Y b4 The stress tensor integral values ​​of place cells showed a decreasing trend, which were 1.99pN / m and 0.82pN / m, respectively. Therefore, when the radius of curvature is 100 μm, it can be b1 ~Yb4 Four locations capture four cells of different volumes, and in Y b2 The position captures the largest cell volume, in Y b4 The location captures the smallest volume of cells.

Embodiment 3

[0035] Example 3 Cell sorting with a bird's beak-shaped fiber optic probe with a radius of curvature of 150 μm

[0036] like Image 6 shown, when the radius of curvature of the bird’s beak fiber probe is 150 μm, Y b5 The gradient force on the position cell is greater than the light scattering force, so it can be trapped at this position, and the integral value of the stress tensor is 0.58 pN / m. while in Y b1 ~Y b4 The stress tensor integral values ​​of place cells showed a decreasing trend, which were 2.20pN / m, 2.19pN / m, 1.09pN / m and 0.38pN / m, respectively. Therefore, when the radius of curvature is 150 μm, it can be b1 ~Y b5 Capture five cells of different volumes at five locations, and in Y b1 The position captures the largest cell volume, in Y b4 The location captures the smallest volume of cells.

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Abstract

A single-fiber core bird's beak-shaped optical fiber optical tweezers system with sorting function is characterized in that the optical fiber optical tweezers is composed of a bird's beak-shaped optical fiber probe, a laser, an adjustment frame, a sample slot and a microscopic imaging system. The bird's beak-shaped optical fiber probe is characterized in that the evanescent field distribution on the side of the optical fiber is changed by adjusting the curvature radius of the bending tip region of the optical fiber to form a differentiated gradient light field. Cells of different volumes are subjected to different gradient forces at different positions in the gradient light field, and the magnitude of the optical trap force is also different, so that the fiber optic tweezers can realize controllable screening of cell volumes.

Description

(1) Technical field [0001] The invention relates to a single-core bird's beak-shaped optical fiber optical tweezers with sorting function and a preparation method thereof, which can be used for cell volume screening and belong to the technical field of optical manipulation. (2) Background technology [0002] Cell sorting is one of the important aspects in the field of cell manipulation. Sorting cells in biochemistry, biophysics, etc. can reduce the difficulty of manipulation, avoid the influence of other particles, and optimize the experimental results. The commonly used cell sorting methods are flow cytometry, immunomagnetic bead method, fluorescence activation method, centrifugation method and laser induction method. [0003] However, the above methods all have certain limitations, such as the screening technology of extracellular vesicle surface protein-specific aptamers based on immunomagnetic beads method (patent number: CN110791504A) and the detection reagents based o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B6/02G02B6/255G21K1/00C12M1/00
CPCG02B6/02G02B6/2552G21K1/006C12M47/04
Inventor 成煜冯智宇申泽苑立波陈明
Owner GUILIN UNIV OF ELECTRONIC TECH