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Bifidobacterium adolescentis and application thereof in metabolic syndrome

A technology for metabolic syndrome and bifidobacteria, applied in the field of bifidobacterium adolescence and its application in metabolic syndrome, can solve the problems of limited number of probiotics, relieve hepatic steatosis, improve glucose tolerance and insulin resistance , the effect of inhibiting the activity

Active Publication Date: 2021-08-13
BEIJING QUANTIHEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of known probiotics is still limited, so the intestinal microecological resources and related functions still need to be excavated and studied

Method used

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  • Bifidobacterium adolescentis and application thereof in metabolic syndrome
  • Bifidobacterium adolescentis and application thereof in metabolic syndrome
  • Bifidobacterium adolescentis and application thereof in metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]Example 1 Screening and identification of Bifidobacterium adolescentis BUAD

[0033] The screening process of Bifidobacterium adolescentis BUAD provided by the invention is as follows:

[0034] 1. Collection of samples

[0035] The feces of healthy adults were collected as screening samples. Before the collection, the population had never taken antibiotics, had no history of taking probiotics, and had no history of gastrointestinal diseases.

[0036] 2. Isolation of strains

[0037] Dilute the collected stool samples and spread them on MRS medium, culture anaerobically at 37°C for 24-48 hours, pick a single colony and streak culture on a new MRS medium plate to obtain purified colonies, and then spread the single colonies On the mass spectrometer plate, the lysate and the matrix were added and dried on the MALDI-TOF MS 1000 mass spectrometer (Autobio, Zhengzhou Antu Biotechnology Co., Ltd.) for identification on the machine. The identification results are as follows: ...

Embodiment 2

[0039] Example 2 Bifidobacterium adolescentis BUAD live bacteria liquid, preparation of metabolites and inactivated bacteria

[0040] 1. Culture of bacteria

[0041] Take -80°C frozen bacteria liquid and spread it on the MRS solid plate, incubate upside down at 37°C for 24-48 hours, take a single colony and inoculate it in the liquid MRS medium, and culture it at 37°C for 18-24 hours to obtain the first-generation bacterial liquid; Take 10% of the first-generation bacterial liquid and inoculate it into fresh MRS liquid medium, and incubate at 37°C for 18-24 hours to obtain the second-generation bacterial liquid; take 10% of the second-generation bacterial liquid and inoculate it into fresh MRS liquid medium, and incubate at 37°C for 18-24 hours to obtain working bacteria.

[0042] 2. Obtaining live bacteria solution

[0043] The live bacterial liquid can also be obtained by other methods in the technical field, as long as the bacterial cells can be enriched from the culture ...

Embodiment 3

[0049] Example 3 Gastrointestinal tolerance experiment of Bifidobacterium adolescentis BUAD

[0050] 1. Determination of tolerance of Bifidobacterium adolescentis BUAD to artificial gastric juice

[0051] 1.1 Preparation of artificial gastric juice

[0052] Accurately measure 20 ml of 1 mol / l hydrochloric acid solution, add distilled water to adjust the pH value of the hydrochloric acid solution to 2.5, then add pepsin (1 g / 100 ml) into the adjusted hydrochloric acid solution, after fully dissolving, use The dissolved pepsin was filtered and sterilized with a 0.22 μm filter membrane to obtain artificial gastric juice.

[0053] 1.2 Tolerance test of strains

[0054] Collect the cultured viable cells and centrifuge at 13,000 rpm at 4°C for 15 minutes, discard the liquid, retain the precipitated cells, and resuspend them with normal saline. 9 CFU / ml was inserted into simulated gastric juice with pH 2.5, cultured at 37°C for 0, 1h, 2h, and 3h, and then samples were taken to det...

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Abstract

The invention provides bifidobacterium adolescentis and application thereof in metabolic syndrome, and relates to the field of microorganisms. The collection number of the bifidobacterium adolescentis is CGMCC No.22147, the bifidobacterium adolescentis is collected in China General Microbiological Culture Collection Center on April 9, 2021, and the collection address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Bifidobacterium adolescentis provided by the invention can promote the glucose consumption of HepG2 hepatoma carcinoma cells, inhibit the activity of alpha-amylase, remarkably reduce the weight and blood fat content of high-fat diet mice, improve glucose tolerance and insulin resistance, relieve liver fatty degeneration, and has important application significance for preventing or treating metabolic diseases such as diabetes, obesity and the like.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a bifidobacterium adolescent and its application in metabolic syndrome. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is a pathological state of abnormal fasting blood sugar, and it is also one of the so-called "three highs". Short-term, one-time hyperglycemia does not cause serious damage to the human body, and sustained high blood sugar levels can cause damage and dysfunction of the heart, kidneys, nerves and other systemic organs. Diabetes is clinically found to be a major cause of blindness, kidney failure, heart attack, stroke, and lower limb amputation. In 2016 alone, approximately 1.6 million people died from diabetes, according to the World Health Organization. At present, the prevalence of diabetes in my country ranks first in the world, and the incidence rate is as high as 10%. Therefore, in order to impr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P3/10A61P1/16A61P3/04A61P3/06A61P9/12A61P19/06A23L33/135C12R1/01
CPCA61K35/745A61P3/10A61P1/16A61P3/04A61P3/06A61P9/12A61P19/06A23L33/135A23V2002/00A23V2400/513A23V2200/328A23V2200/332A23V2200/3262A23V2200/326A23V2200/30
Inventor 徐文艺赵柏闻李转羽齐桂红张虹璇
Owner BEIJING QUANTIHEALTH TECH CO LTD
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