Method for simultaneously detecting Hg2+ and Ag+ based on single excitation

A nucleic acid and molecular technology, which is applied in the field of preparation of nanocapsule-nucleic acid biomolecular complexes, can solve problems that have not been reported in the literature, and achieve the effects of wide application range, improved detection sensitivity, and high selectivity detection

Pending Publication Date: 2021-08-13
QINGDAO UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

So far, single-excitation-based, nanocapsule-nucleic acid biomolecule complexes have been used for the simultaneous detection of Hg 2+ and Ag + technology has not yet been reported in the literature

Method used

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  • Method for simultaneously detecting Hg2+ and Ag+ based on single excitation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] An HG is detected while preparing a single excitation proposed by the present invention 2+ And AG + Based on nucleic acid extracormid enzyme circulation amplifier technology and biomolecular identification technology, nano-capsule-nucleic acid biomolecular complex, including the following steps:

[0031] (1) By selecting the different types and the number of bases, two nucleic acid biological recognition molecules are designed and synthesized, respectively, which are respectively paired and only for the target metal ion Hg. 2+ Or AG + There is a specific response, and there is no response to any other coexistence ions and interference ions;

[0032] (2) After washing 20 μl of magnetic beads with pH = 7.4, 20 μl of nano-gold carrier solution having hollow and porous structures was added, and 200 μl of 11.664 mg / ml of polydienne dimethyl chloride solution, 37 The magnetic separation was magnetically separated from the ° C for 10 h, and was washed with a MOPS buffer solution...

Embodiment 2

[0040] SINIC-based detection HG 2+ And AG + The method is as follows:

[0041] (1) 20 μL containing HG 2+ And AG + Solution to the present invention to detect Hg simultaneously 2+ And AG + In the nano-capsule-nucleic acid biomolecular complex, 1 μL of EXOIII shear enzyme (20U) was added, and the reaction temperature was 37 ° C, which was 37 ° C for Hg. 2+ And AG + The presence, the nucleic acid biomolecule assembled to the surface of the nanocapsules respectively 2+ And AG + The specific response occurs, the two ions specifically bind to their respective nucleic acid biomolecules, respectively, to change from the surface of the nanoculate surface, so that the blocking hole is opened, resulting in RHB and CY5 from nanocapsules The interior is released; at the same time, since the shear effect of the erythrarase EXOIII, the generated binding structure has a specific binding product of the generation to release Hg. 2+ And AG + And the release of HG 2+ And AG + It is possible to ident...

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Abstract

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method for simultaneously detecting Hg2+ and Ag+ based on single excitation, the method utilizes a single excitation technology and adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, not only can realize simultaneous detection of Hg2+ and Ag+, but also obviously improves the detection sensitivity on the premise of ensuring high specificity, and meanwhile, obtains a wider linear detection range on Hg2+ and Ag+.

Description

Technical field [0001] The present invention relates to HG 2+ And AG + At the same time, detect, specifically, a single-discipline-based detection HG 2+ And AG + Preparation method of nano capsule - nucleic acid biomolecular complex and its application thereof, belonging to environmental monitoring and nano-biotechnology. Background technique [0002] Mercury ion (Hg 2+ ) And silver ions (AG + ) Two kinds of metal ions with severe toxicity are also widely distributed, even if low concentrations constitute a serious threat, resulting in serious, permanent damage. More seriously, by contaminated water, HG 2+ And AG + You can continue to accumulate in agricultural products and water products, entering human food chains. If it is exposed to HG for a long time 2+ And AG + In the contaminated environment, it can cause the epitaxial disease of the human body and the neurological degenerative disease. To this end, the World Health Organization (WHO) and the US Environmental Protection Ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/53
CPCG01N21/6486G01N33/5308
Inventor 王卫李欣唐凯董传诚宋志灵罗细亮
Owner QINGDAO UNIV OF SCI & TECH
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