Method for simultaneously detecting Hg2+ and Ag+ based on single excitation

A nucleic acid and molecular technology, which is applied in the field of preparation of nanocapsule-nucleic acid biomolecular complexes, can solve problems that have not been reported in the literature, and achieve the effects of wide application range, improved detection sensitivity, and high selectivity detection
CN113252622APending Publication Date: 2021-08-13QINGDAO UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO UNIV OF SCI & TECH
Publication Date
2021-08-13

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Abstract

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method for simultaneously detecting Hg2+ and Ag+ based on single excitation, the method utilizes a single excitation technology and adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, not only can realize simultaneous detection of Hg2+ and Ag+, but also obviously improves the detection sensitivity on the premise of ensuring high specificity, and meanwhile, obtains a wider linear detection range on Hg2+ and Ag+.
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Description

Technical field

[0001] The present invention relates to HG 2+ And AG + At the same time, detect, specifically, a single-discipline-based detection HG 2+ And AG + Preparation method of nano capsule - nucleic acid biomolecular complex and its application thereof, belonging to environmental monitoring and nano-biotechnology. Background technique

[0002] Mercury ion (Hg 2+ ) And silver ions (AG + ) Two kinds of metal ions with severe toxicity are also widely distributed, even if low concentrations constitute a serious threat, resulting in serious, permanent damage. More seriously, by contaminated water, HG 2+ And AG + You can continue to accumulate in agricultural products and water products, entering human food chains. If it is exposed to HG for a long time 2+ And AG + In the contaminated environment, it can cause the epitaxial disease of the human body and the neurological degenerative disease. To this end, the World Health Organization (WHO) and the US Environmental Protection Ag...

Claims

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