Methods of producing two chain proteins in prokaryotic host cells

A host cell, protein technology, applied in the field of prokaryotic host cells, can solve the problem of increasing complexity of recombinant therapy

Pending Publication Date: 2021-08-13
F HOFFMANN LA ROCHE & CO AG
View PDF77 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As molecular biology tools and knowledge advance, so does the complexity of recombinant therapies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of producing two chain proteins in prokaryotic host cells
  • Methods of producing two chain proteins in prokaryotic host cells
  • Methods of producing two chain proteins in prokaryotic host cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0297] The present disclosure will be understood in more detail with reference to the following examples. However, they should not be construed as limiting the scope of the present disclosure. It should be understood that the examples and embodiments described herein are for illustrative purposes only, and that various modifications or changes thereof will be suggested to those skilled in the art, and will be included within the spirit and scope of this application and the scope of the appended claims. within range.

example 1

[0298] Example 1: Engineering E. coli strains with chromosomally integrated promoters controlling the expression of the chaperone proteins DsbA, DsbC and FkpA

[0299] Overexpression of the chaperone proteins DsbA, DsbC and FkpA from plasmids improves the production of antibody-based products in bacterial culture. However, expression of these chaperone proteins from plasmids has several disadvantages. For example, this approach requires the development and regulation of expression plasmids for each new product. In some cases, large plasmid sizes can also lead to lower product titers. Additionally, plasmids are typically present in 10-15 copies per cell, resulting in high levels of overexpression, which may require downstream purification steps to remove chaperone proteins (eg, FkpA) from the product. In some cases, lower expression levels of one or more chaperone proteins can achieve the same product titers.

[0300] Here, the native promoters of dsbA, dsbC, and fkpA in the...

example 2

[0321] Example 2: Overexpression of FkpA under the control of a chromosomally integrated promoter

[0322] Overexpression of FkpA was compared in strains engineered as described in Example 1 with an integrated promoter overexpressing native FkpA compared to strains carrying a plasmid expressing FkpA.

[0323] To assess the ability of FkpA to be overexpressed in the chromosome, the native promoter of FkpA was exchanged with the tac, phoA or CP25 promoter according to the method of Example 1. The lacI promoter was also modified to further enhance the strength of the tac promoter. Strains were grown in 10 L fermentations and FkpA production was assessed by Western blotting.

[0324] Chromosomal expression driven by the CP25 promoter showed the highest level of FkpA expression, followed by expression driven by the phoA promoter ( figure 2 ). As expected, the tac promoter showed the lowest level of FkpA expression, with higher FkpA expression in the ΔlacI background than in the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Provided herein are methods and host cells for producing a polypeptide containing two chains, such as an antibody, half-antibody, antibody fragment, or one-armed antibody. The methods and host cells allow for two-chain polypeptide production using expression of polynucleotides encoding the polypeptide chains from extra-chromosomal polynucleotide(s), and expression of one or more chaperone protein(s) (e.g., peptidyl-prolyl isomerases and/or protein disulfide oxidoreductases) from the host cell chromosome using non-native combination(s) of promoters and translational units encoding a chaperone protein.

Description

[0001] Cross references to related patent applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 755,915, filed November 5, 2018, each of which is incorporated herein by reference in its entirety. [0003] Submit a sequence listing as an ASCII text file [0004] The contents of the following submitted ASCII text file are hereby incorporated by reference in their entirety: Sequence Listing in Computer Readable Format (CRF) (file name: 146392044040SEQLIST.TXT, date of record: October 28, 2019, size: 6KB). technical field [0005] The present disclosure relates to methods of producing recombinant polypeptides, such as antibodies (eg, bispecific antibodies, half antibodies, one-armed antibodies, antibody fragments, etc.), and prokaryotic host cells that may find use in such methods. Background technique [0006] Since the production of human insulin in E. coli in 1978, the production of recombinant proteins in prokaryotic host cells has be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00C07K16/24C12N9/90C12N15/70
CPCC07K16/00C12N15/70C07K16/244C07K2317/14C07K2317/31C12N9/90C12Y502/01008C07K16/2863C07K16/22C07K2317/24C07K2317/55C07K2317/92C12N9/0004
Inventor K·维拉瓦利R·麦肯纳
Owner F HOFFMANN LA ROCHE & CO AG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap