Preparation method and application of self-assembled nano-drug liposome for resisting Alzheimer's disease

A technology of Alzheimer's disease and nano-medicine, which is applied in the field of medicine, can solve the problems of poor therapeutic effect and low blood-brain barrier penetration rate, and achieve low cost, huge economic and social benefits, and enhanced effects

Active Publication Date: 2021-08-17
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application thereof of self-assembled nano drug liposomes for anti-Alzheimer's disease, which can effectively solve the problem of traditional treatment of Alzheimer's disease. The problem of low blood-brain barrier penetration rate and poor therapeutic effect of anti-inflammatory drugs

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  • Preparation method and application of self-assembled nano-drug liposome for resisting Alzheimer's disease

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Effect test

Embodiment 1

[0015] A kind of preparation method of the self-assembled nano drug liposome of anti-Alzheimer's disease of the present invention, comprises the following steps:

[0016] (1) Dissolve 0.3 mg of the hydrophobic anti-inflammatory drug tea polyphenol in 1 mL of absolute ethanol, then add it into 5 mL of water, and stir vigorously for 3 minutes to obtain the self-assembled hydrophobic anti-inflammatory nanoparticle drug;

[0017] (2) Dissolve 5mg dioleoylphosphatidylcholine, 5mg distearoylphosphatidylcholine, 0.4mg cholesterol, 0.8mg T7 peptide-polyethylene glycol-phosphatidylethanolamine in 1mL chloroform to form a solution , add the solution to 4 mL of chloroform, distill under reduced pressure on a rotary evaporator at 40°C to form a thin film on the inner wall of the eggplant-shaped bottle, continue rotary evaporation for 3 minutes, and add 3 mL of self-assembled hydrophobic anti-inflammatory nanoparticle drug , the film was dispersed, and then probed under the condition of 20...

Embodiment 2

[0019] A kind of preparation method of the self-assembled nano drug liposome of anti-Alzheimer's disease of the present invention, comprises the following steps:

[0020] (1) Dissolve 2.4 mg of curcumin, a hydrophobic anti-inflammatory drug, in 2 mL of absolute ethanol, then add it to 15 mL of water, and stir vigorously for 4 minutes to obtain a self-assembled hydrophobic anti-inflammatory nanoparticle drug;

[0021] (2) 8 mg dioleoyl phosphatidyl choline, 8 mg distearoyl phosphatidyl choline, 0.6 mg cholesterol, 1.6 mg T7 peptide-polyethylene glycol-phosphatidylethanolamine were dissolved in 1.5 mL chloroform to form solution, add the solution to 8 mL of chloroform, distill under reduced pressure on a rotary evaporator at 40°C to form a thin film on the inner wall of the eggplant-shaped bottle, continue to rotate for 4 minutes, and add 4 mL of self-assembled hydrophobic anti-inflammatory nanoparticles For the drug, the thin film is dispersed, and then probed under the conditi...

Embodiment 3

[0023] A kind of preparation method of the self-assembled nano drug liposome of anti-Alzheimer's disease of the present invention, comprises the following steps:

[0024] (1) Dissolve 4.4 mg of the hydrophobic anti-inflammatory drug indomethacin in 4 mL of absolute ethanol, then add it to 20 mL of water, and stir vigorously for 5 minutes to obtain a self-assembled hydrophobic anti-inflammatory nanoparticle drug;

[0025] (2) 10 mg dioleoyl phosphatidyl choline, 10 mg distearoyl phosphatidyl choline, 1.0 mg cholesterol, 2.0 mg T7 peptide-polyethylene glycol-phosphatidylethanolamine were dissolved in 2 mL chloroform to form a solution, Add the solution to 10 mL of chloroform, and distill it under reduced pressure at 40°C on a rotary evaporator to form a thin film on the inner wall of the eggplant-shaped bottle. Continue rotary evaporation for 5 min, add 5 mL of self-assembled hydrophobic anti-inflammatory nanoparticle drug, The thin film is dispersed, and then probed under the c...

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Abstract

The invention relates to a preparation method and application of self-assembled nano-drug liposome for resisting Alzheimer's disease, and can effectively solve the problems of low blood-brain barrier penetration rate and poor treatment effect of the traditional Alzheimer's disease treatment medicine. The preparation method comprises the following steps: dissolving a hydrophobic anti-inflammatory drug in absolute ethyl alcohol, adding into water, and violently stirring to obtain the self-assembled hydrophobic anti-inflammatory nano particle drug; and dissolving dioleoyl phosphatidylcholine, distearoyl phosphatidylcholine, cholesterol and T7 peptide-polyethylene glycol-phosphatidyl ethanol amine in trichloromethane to form a solution, adding the solution into trichloromethane, carrying out reduced pressure distillation to form a thin film, continuously carrying out rotary evaporation, adding the self-assembled hydrophobic anti-inflammatory nano particle drug, dispersing the thin film, and carrying out ultrasonic detection to obtain the self-assembled nano-drug liposome for resisting the Alzheimer's disease. The preparation method is stable, reliable and low in cost, plays a huge role in preparing the medicine for treating the Alzheimer's disease, enhances the effect of treating the Alzheimer's disease, and is an innovation of the medicine for treating the Alzheimer's disease.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of an anti-Alzheimer's disease self-assembled nano drug liposome, which is a nano drug delivery system capable of delivering anti-inflammatory drugs to brain lesions. Background technique [0002] Alzheimer's disease (AD) is a chronic, multifactorial neurodegenerative disease, mainly characterized by progressive cognitive impairment and memory loss, which brings a huge burden to individuals, families, and society . As the innate immune cells in the central nervous system, microglia are crucial to maintaining the health of the brain and play a key role in the occurrence and development of AD. Microglia are divided into two phenotypes, the M1 (pro-inflammatory) type promotes the secretion of neurotoxic β-amyloid (Aβ) from neurons, while the M2 (anti-inflammatory) type distributes neurotrophic factors to damaged neurons And anti-inflammatory factors, in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/192A61K31/12A61K31/405A61K31/353A61K47/24A61K47/28A61P25/28
CPCA61K9/1277A61K31/192A61K31/12A61K31/405A61K31/353A61K47/24A61K47/28A61P25/28
Inventor 冯倩华王蕾张雪莉梅玉影袁孝敏
Owner ZHENGZHOU UNIV
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