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Sperm SP10 protein detection test strip and quantitative detection method

A protein detection and test strip technology, applied in the field of instant detection, can solve the problems of inability to accurately quantify the concentration of SP10 protein, affect the accurate detection of sperm concentration, and the high price of commercial products, and achieve low cost, fast detection, false positives and The effect of low probability of false negative

Pending Publication Date: 2021-08-17
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing SP10 protein test strips (David Sperm SP10 Protein Test Strip, Kim Xiuer Sperm SP10 Protein Test Reagent, FertilMARQ Fertility Sperm Test Kit, Spermcheck Fertility Home Test Kit, and BabystartFertilCount Male Fertility Test) are all based on colorimetric methods for identification. Only the qualitative identification of SP10 protein can be carried out, and the concentration of SP10 protein cannot be accurately quantified, which affects the accurate detection of sperm concentration; The price of a single root is about 40 yuan. Therefore, it can be seen that the prices of existing commercial products are generally high, which is not conducive to the promotion and use in poor areas.

Method used

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  • Sperm SP10 protein detection test strip and quantitative detection method

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Effect test

Embodiment 1

[0042] see figure 1 The technical solution provided by the present invention is a test strip for quantitative detection of sperm SP10 protein based on SERS, comprising the following steps:

[0043] A) Construction of lateral flow test strips: substrate 1 is made of PVC board, its length is 40 mm, and its width is 2 mm; sample pad 2, marker pad 3, chromatographic membrane 4 and Absorbent pad 5, wherein the sample pad is made of glass fiber membrane with a length of 10 mm and a width of 2 mm; the material of the marker pad is a glass fiber membrane with a length of 6 mm and a width of 2 mm; the material of the chromatography membrane is a nitrocellulose membrane, The length is 20mm and the width is 2mm; the absorbent pad is made of absorbent paper, the length is 10mm, and the width is 2mm; the detection area 6 is a detection line drawn by using the SP10 capture antibody; the quality control area 7 is the detection line using the SP10 detection antibody. A quality control line d...

Embodiment 2

[0052] Fluorescence-based sperm SP10 protein quantitative detection test strips, comprising the following steps:

[0053] A) Construction of lateral flow test strips: substrate 1 is made of PDMS plate with a length of 198 mm and a width of 6 mm; sample pad 2, marker pad 3, chromatographic membrane 4 and Absorbent pad5. The material of the sample pad is nylon film with a length of 40 mm and a width of 6 mm; the material of the marker pad is non-woven fabric with a length of 10 mm and a width of 6 mm; the material of the chromatography membrane is cellulose acetate film with a length of 80 mm and a width of 6 mm. The material of the absorbent pad is filter paper, the length is 80mm, and the width is 6mm. The detection area 6 is a single detection point composed of an aptamer corresponding to the SP10 protein; the quality control area 7 is a single quality control point composed of an aptamer corresponding to the SP10 aptamer. The quality control point is on the right side of t...

Embodiment 3

[0062] A test strip for quantitative detection of sperm SP10 protein based on a magnetic signal, comprising the following steps:

[0063] A) Construction of lateral flow test strips: substrate 1 is made of PS plate, its length is 139 mm, and its width is 4 mm; sample pad 2, marker pad 3, chromatographic membrane 4 and Absorbent pad 5, wherein the material of the sample pad is a non-woven film, the length is 20 mm, and the width is 4 mm; the material of the marking pad is polyvinyl acetate film, the length is 8 mm, and the width is 4 mm; the material of the chromatographic membrane is polyvinylidene fluoride , with a length of 60mm and a width of 4mm; the absorbent pad is made of cellulose-glass fiber composite, with a length of 60mm and a width of 4mm. The detection area 6 is a detection matrix composed of ligands corresponding to the SP10 protein; the quality control area 7 is a quality control lattice composed of ligands corresponding to the SP10 ligands. The quality contro...

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Abstract

The invention provides a sperm SP10 protein detection test strip and a quantitative detection method, and belongs to the technical field of instant detection. According to the technical scheme, the detection test strip is arranged in a lateral flow mode, a substratum serves as a substrate, and a sample pad, a marking pad, a chromatography membrane and a water absorption pad are sequentially arranged on the substrate; a detection area and a quality control area are arranged on the chromatography membrane, and a functionalized nano material capable of being combined with SP10 protein is attached to the marking pad; and the two adjacent structures are partially overlapped. The test strip has the advantages that the test strip is used for quantitatively detecting the concentration of male sperms, a sample solution containing SP10 protein is directly dropwise added to the sample pad, quantitative detection of the concentration of the SP10 protein is achieved through signal collection and analysis of the detection area, and the test strip has the advantages of being easy and convenient to operate, low in cost, rapid in detection and the like; wide application prospects are realized in the field of male reproduction.

Description

technical field [0001] The invention relates to the technical field of instant detection, in particular to a sperm SP10 protein detection test strip and a quantitative detection method. Background technique [0002] According to the "Human Semen Examination and Processing Laboratory Manual", the main factors that cause male infertility include semen volume, total number of sperm, sperm concentration, normal sperm morphology, semen pH value and liquefaction time, etc. Among them, the sperm concentration is An important indicator to measure male infertility, when the concentration of sperm is less than 15×10 6 Individuals / mL, it is considered to be difficult to reproduce. Based on anatomical, genetic, and biochemical features, the SP10 protein, which has been shown to be useful as a biomarker of sperm concentration in men, is expressed only in the testis and is a marker of differentiation during the final step in spermatogenesis. According to the research, the concentration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N33/52
CPCG01N33/689G01N33/558G01N33/52G01N2800/367G01N2333/47
Inventor 刘兵孙斐
Owner NANTONG UNIVERSITY
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