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A method for cultivating algal crusts in calcareous sandy soil of coral islands and reefs in the South China Sea

A cultivation method and sandy soil technology are applied in the field of artificial cultivation of algal crusts in calcareous sandy soil, and achieve the effects of retaining microbial diversity, fast cultivation speed and good adaptability

Active Publication Date: 2022-08-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical status of the successful cultivation of artificial algae fungus crusts in the environment of typical tropical coral islands and reefs in the South China Sea, the present invention aims at the extremely hot and rainy summer conditions of typical tropical coral islands and reefs in the South China Sea, and the dry and relatively hot conditions in spring and winter. According to the principle that biodiversity can maintain the stability of the ecosystem to the greatest extent, the diversity of algal species in biological crusts is an important basis for ensuring the healthy development of biological crusts on artificial calcareous sandy soils

Method used

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  • A method for cultivating algal crusts in calcareous sandy soil of coral islands and reefs in the South China Sea
  • A method for cultivating algal crusts in calcareous sandy soil of coral islands and reefs in the South China Sea
  • A method for cultivating algal crusts in calcareous sandy soil of coral islands and reefs in the South China Sea

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Optimization of artificial cultivation conditions and technical construction of algal crusts in typical tropical coral islands and reefs in the South China Sea

[0028] The invention can ensure that the seed soil derived from the typical tropical coral island reef algae crust of the South China Sea can use carbon source, nitrogen source, phosphorus source, and different concentrations of potassium, magnesium and iron in the simulated South China Sea island reef natural environment. A method of artificially cultivating algal crusts with high biomass by comparing and combining the optimum inoculum amount in indoor cultivation. The specific operation is as follows:

[0029] 1. Consider the seven factors of inoculum size, glucose, sodium nitrate, disodium hydrogen phosphate, potassium chloride, magnesium chloride and ferrous chloride, and set 4 levels for each factor, and then use the orthogonal table of seven factors and four levels to get a total of 32 There a...

Embodiment 2

[0040] Example 2: Indoor inspection of the effect of artificial cultivation technology of algal crusts on tropical coral islands and reefs in the South China Sea

[0041] During the experiment, the biomass of the algal crust samples was measured at the beginning and end of the experiment, respectively. The specific measurement method is:

[0042] 1) Put it into a freeze dryer to remove moisture (48h) before measurement;

[0043] 2) Take out the crust, remove the loose sand, and record the thickness of the crust with a vernier caliper (uniform thickness standard, about 0.1cm), and then grind it with a mortar in the dark to separate the algae from the sand, and disperse the algae;

[0044] 3) Weigh 2g (±0.02g) of the samples respectively and put them in a stoppered graduated test tube, extract and measure the content of chlorophyll a in it with acetone (represented by the content of chlorophyll a in each gram of dry crusted soil sample), and set 3 replicates. ;

[0045] 4) A...

Embodiment 3

[0050] Example 3: Field test of the effect of artificial cultivation technology of algal crusts on tropical coral islands and reefs in the South China Sea

[0051] Utilize the algal bacterial crusts artificially cultivated by the combination of 3 methods in Example 1, and the fresh algal bacterial crust samples collected in the tropical coral reefs of the South China Sea, respectively, make seed soil, and inoculate both in the tropical coral reef environment to carry out artificial The adaptability test of cultivated algal crusts in the field. The specific operation method is as follows:

[0052] 1) Using a soil pulverizer, the algal bacterial crusts cultivated in the laboratory and the fresh algal bacterial crusts collected are evenly pulverized, respectively, to make three types of crusted seed soils, namely: artificial algal bacterial crusts seed soil, in situ Fresh algal crust seed soil and mixed seed soil with the two evenly mixed in a ratio of 1:1;

[0053] 2) The thre...

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Abstract

The invention discloses a method for cultivating algal bacterial crusts suitable for the calcareous sandy soil of coral islands and reefs in the South China Sea. The method directly propagates and domesticates the in-situ algal bacterial crust, and uses the cultivated algal bacterial crust for direct field inoculation, which saves the process of separation and purification, and the cultivation speed is fast, which saves the amount of materials used. And improve the field adaptability of artificially cultivated algal crusts, which has important application value to the ecosystem restoration of coral reefs in the South China Sea.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of algal bacterial crusts in calcareous sandy soils, and particularly relates to a method for cultivating algal bacterial crusts suitable for calcareous sandy soils of coral islands and reefs in the South China Sea. Background technique [0002] Biological soil crusts (biocrusts) are microscopic particles on the soil surface formed by bacteria, cyanobacteria, diatoms or green algae, microscopic Fungi, lichens, and bryophytes, as well as non-vascular plant components common to many landscapes, utilize complex complexes formed by the "cement" of mycelium, rhizomes, and exudates. The roles and functions of biological crusts in desert ecosystems, such as the ecological roles exhibited by soil ecological processes, biogeochemical cycle processes, ecological processes, and hydrological processes, have been gradually recognized in recent years. It has been concretely applied in the practi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01B79/00C12N1/14
CPCA01B79/00C12N1/14
Inventor 张偲王琳李洁
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI