Application of egta in the ability of calcium chloride to enrich calcium and reduce cadmium in the culture of shiitake mushroom
A technology of calcium chloride and cadmium chloride, applied in the fields of application, mushroom cultivation, cultivation, etc., can solve the problems affecting the output and quality of shiitake mushrooms, the damage of human immunity to liver and kidney, and the growth of shiitake mushrooms, so as to reduce the content and ensure safety sex, reducing toxicity
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[0028] 2.1 Preparation of mushroom mycelium
[0029] Under the condition of 25°C and 150 r / min, the strains were cultured in maltose medium on a shaker for 20 days for activation. At the time of inoculation, the cadmium concentration in all treatment groups was brought to 2 mg / L, and then the medium containing 12 mmol / L EGTA and 10 mg / L Ca, 20 mg / L Ca, 50 mg / L Ca, 70 mg / L Ca, 100 mg / L Ca and 150 mg / L Ca (10 mg / L Ca+EGTA is 2 mg / L Cd+10 mg / L Ca+12 mmol / L EGTA, 20 mg / L Ca+EGTA is 2 mg / L Cd+20 mg / L Ca+12 mmol / L EGTA, 50 mg / L Ca+EGTA as 2 mg / LCd+50 mg / L Ca+12 mmol / LEGTA, 70 mg / L Ca+EGTA as 2 mg / L Cd+70 mg / L Ca+12 mmol / L EGTA, 100 mg / L Ca+EGTA to 2 mg / L Cd+100 mg / L Ca+12 mmol / L EGTA, 150 mg / L Ca+EGTA (2 mg / L Cd+150 mg / L Ca+12 mmol / L EGTA treatment group), cultured on a shaker for 20 days at 25°C and 150 r / min. The mycelia were then harvested by centrifugation and washed three times with deionized water. The washed mycelia were subjected to the next experimental operation or store...
Embodiment 1
[0041] Sampling 215 varieties of shiitake mushrooms, adding 2 mg / L of cadmium chloride and 10 mg / L, 20 mg / L, 50 mg / L, 70 mg / L, 100 mg / L and 150 mg to the malt extract powder liquid medium The effect of / L Ca+EGTA on the growth of Lentinus edodes mycelium, the results are shown in figure 1 .
[0042] Depend on figure 1 It can be seen that the mycelium of the CK group grows normally, and the amount of mycelium in the Cd treatment group is significantly less than that in the other groups, indicating that Cd does poison the mycelium of Lentinus edodes and affects its normal growth. The hyphae of the CK+EGTA and Cd+EGTA treatment groups did not grow, probably because EGTA entered the body without adding exogenous calcium, and the addition of EGTA chelated the endogenous calcium of the Lentinus edodes hyphae, and the hyphae were seriously deficient in nutrition. Elements lead to the death of mycelium. Some studies have found that adding EGTA alone can significantly increase the to...
Embodiment 2
[0060] Sampling 215 varieties of Lentinus edodes, add 2 mg / L cadmium chloride to the malt extract powder liquid medium (common medium), then add 20 mg / L calcium chloride and 12 mmol / L EGTA to the medium, and use malt Extraction powder liquid medium, malt extraction powder medium supplemented with cadmium chloride, malt extraction powder medium supplemented with EGTA, malt extraction powder liquid medium supplemented with cadmium chloride and EGTA as controls, cultured for 20 days, digested with microwave- Determination of cadmium content in mushroom mycelium by inductively coupled plasma mass spectrometry.
[0061] from Image 6 It can be seen that the hyphae in the normal state ( Image 6 A) Compared with the addition of cadmium chloride, the mushroom mycelium ball is larger, but the amount is less ( Image 6 B), which means that cadmium will poison the mushroom mycelium and affect the normal growth of the mushroom mycelium; only a few mushroom mycelium can be seen after ad...
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