Cell composition comprising liver progenitor cells expressing hla-e

A technology of HLA-E and composition, applied in the direction of liver cells, drug combinations, animal cells, etc., can solve the problems of not disclosing liver stem cells or progenitor cell populations, not mentioning HLA-E expression, etc., to increase efficacy and safety Sexual, potentiated immunosuppressive and/or immune-evading properties

Pending Publication Date: 2021-08-27
PROMETHERA THERAPEUTICS SA
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0010] None of these prior art documents disclose the need to obtain a population of HLA-E expressing hepatic stem or progenitor cells for clinical use, nor does it mention that sufficient HLA-E expression may play a role in evading cytolysis

Method used

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  • Cell composition comprising liver progenitor cells expressing hla-e
  • Cell composition comprising liver progenitor cells expressing hla-e
  • Cell composition comprising liver progenitor cells expressing hla-e

Examples

Experimental program
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Embodiment

[0207] 1. Preparation of pretreated HLA-E positive hepatic progenitor cells or stem cells

[0208] (a) Preparation of hepatic progenitor cells

[0209] Human adult hepatic progenitor cells were isolated from the liver of healthy cadaver or beating donors as described in EP3140393 or WO2017149059.

[0210] Briefly, hepatocyte preparations were resuspended in Williams'E medium supplemented with 10% FBS, 10 mg / ml INS, 1 mM DEX. primary cells in Flasks were cultured at 37°C in 5% CO 2 cultured in a completely humid atmosphere. After 24 hours, the medium was changed to eliminate non-adherent cells, and refreshed twice a week thereafter, and the cultures were observed daily under the microscope. After 12-16 days, change the medium to high glucose DMEM supplemented with 9% FBS. Cell types with mesenchymal-like morphology emerge and proliferate. When reaching 70-95% confluence, cells were trypsinized with recombinant trypsin and 1 mM EDTA and incubated at 1-10 x 10 3 cells / c...

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PUM

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Abstract

The current invention concerns isolated liver progenitor cells, cell lines thereof, cell populations comprising such and compositions comprising such wherein the liver progenitor cells are HLA-E positive. In addition, the invention concerns methods of preparing these liver progenitor cells.

Description

technical field [0001] The technical field to which the present invention pertains is isolated hepatic progenitor or stem cells derived from the liver and their use in medicine, hepatology, transplantation, liver failure and fibroinflammatory liver disease. Background technique [0002] The liver is a key organ that performs many important functions. Impairment of one of several liver functions can have significant health consequences. The global incidence of acute or chronic liver disease makes these conditions the fifth to ninth leading cause of death according to the World Health Organization. Liver cell transplantation (LCT) is an ongoing clinical investigation procedure for the treatment of liver disease with the goal of liver repair / regeneration (Najimi et al, Stem Cells Translational Medicine, 2016). Despite efforts to make the donor as closely as possible immunologically matched to the recipient, issues related to immune system rejection after hepatocyte transplant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0672C12N2501/2301C12N2501/231C12N2501/24C12N2501/25A61K35/407A61P1/16
Inventor J-L·齐林格里安E·科里托雷G·马扎N·贝尔蒙特E·索卡尔
Owner PROMETHERA THERAPEUTICS SA
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