Tissue culture breeding method for continuous cropping resistant apple rootstocks

Abstract

The invention relates to the technical field of apple tissue culture breeding, and particularly discloses a tissue culture breeding method for continuous cropping resistant apple rootstocks. The method is implemented by equipment comprising base tables, culture dishes, an electric drive pusher, a supporting frame, illumination lamps, a water supply mechanism, an oxygen supply mechanism, leaf surface humidity sensors, plant stem flow sensors, a humidity sensor, PH value sensors, soil moisture content sensors, soil nitrogen phosphorus potassium sensors, a temperature sensor, a displacement sensor, a control panel, a display screen, execution buttons and indicator lamps. According to the method, data of continuous cropping resistant apple seedling bodies, rootstock bodies and growth environments in the culture dishes of the base tables are detected and collected, timely water supply and nutrient supply operation is performed according to data definition, formulation analysis and signal generation, and the growth condition of the continuous cropping resistant apple seedlings in the next stage is predicted, so that the large-scale tissue culture breeding of the continuous cropping resistant apple seedlings is realized, the labor intensity of seedling culture personnel is reduced, and the industrial seedling culture management efficiency is also greatly improved.

Description

technical field [0001] The invention relates to the technical field of apple tissue culture and breeding, in particular to a method for tissue culture and breeding of apple rootstocks resistant to repeated cropping. Background technique [0002] Rootstock refers to the plant that bears the scion when the plant is grafted and propagated. It can fix and support the cuttings, and fit and heal with the scion, so as to promote the later growth and fruiting of the plant. The rootstock can be the whole fruit tree, or the root segment or branch of the tree. The root is the basis for grafting seedlings of fruit trees. The rootstock used for grafting should choose seedlings that grow robustly, have well-developed root systems, have certain resistance, and have a strong affinity with the scion; [0003] In order to solve the problem of repeated cropping of apple trees, apple trees that resist heavy cropping often use rootstock groups to breed seedlings. However, it is difficult to achi...

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Problems solved by technology

[0003] In order to solve the problem of repeated cropping of apple trees, apple trees that resist heavy cropping often use rootstock groups to breed seedlings. However, it is difficult to achieve large-scale and assembly-line breeding for existing anti-heavy apple rootstock groups. Observe the surface changes of apple seedlings under repeated cropping, and then judge their growth conditions. The data generated by the judgment is relatively lagging and imprecise, so it is impossible to provide timely water and nutrient supply for apple seedlings resistant to heavy cropping, and it is also unable to resist Forecast the growth of apple seedlings in double cropping in advance;

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