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Polypeptides, compositions and applications thereof

A composition, peptide coupling technology, applied in the directions of drug combinations, specific peptides, connective tissue peptides, etc., can solve the problems of inability to exert a good therapeutic effect, the complexity of sepsis, etc., to improve bioavailability, small molecular weight, Fast absorption effect

Active Publication Date: 2022-04-29
北京鲲达宇科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical treatment of sepsis mainly relies on antibiotics and other adjuvant treatment measures, but these methods often cannot play a good therapeutic role due to drug resistance, antibiotic toxicity and the complexity of sepsis.

Method used

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  • Polypeptides, compositions and applications thereof
  • Polypeptides, compositions and applications thereof
  • Polypeptides, compositions and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] The step-by-step separation of embodiment 1 collagen 1 active ingredient

[0101] (1) First, dissolve donkey collagen 1 (Advanced Biomatrix product number: 5005) in deionized water at 50°C to obtain a collagen solution, cool to 37°C, and adjust the pH value of the collagen solution to 2.0 (it is recommended to use a pH agent for accurate and adjust the pH value with 1N dilute hydrochloric acid), add pepsin (Sigma company, product number EC3.4.23.1) according to the weight ratio of pepsin and collagen solution of 1:250, under the condition of 37 ℃ , digested for 2 hours; then the pH value of the resulting mixture was adjusted to 6.8, and trypsin (Sigma company, product number EC3.4.21.4) was also added at 37 Digest at ℃ for 2h. Subsequently, the collagen solution digested with pepsin and trypsin was heated to 100°C to inactivate the enzyme for 1 hour, and after the solution was naturally cooled, it was centrifuged at 8000 rpm for 10 minutes to remove precipitated impuri...

Embodiment 2

[0109] Example 2 Ion-exchange chromatographic separation of anti-cell death component A

[0110] (1) Component A (molecular weight ≥ 10,000Da) is separated step by step by ion exchange chromatography, and ion exchange chromatography (IEX) uses IEX technology to purify biomolecules and separate them according to the difference in their surface net charges. This application uses AKTA-pure (GE Healthcare, U.S.) equipped with High-Performance Q (GE healthcare, U.S.) anion exchange column (100mm*10mm Sepax, U.S.). Elution was performed at room temperature with a constant gradient of 50% 1 M NaCl, 50 Mm Tris pH 7.4 at a flow rate of 3 ml / min. Absorbance was monitored at 214 and 280 nm. Fractions from the two collected peaks of A1 and A2 were permeabilized and then lyophilized. The lyophilized solution was dissolved in ultrapure water for analysis. For analysis results, see figure 2 a.

[0111] (2) Detection of the protective effect of the two components A1 and A2 on hydrogen p...

Embodiment 3

[0113] Embodiment 3A1 component is further separated by reverse phase chromatography

[0114] (1) The A1 component is separated step by step by reverse phase chromatography, and the fraction is separated by RPC according to the hydrophobicity of the substance. This application uses AKTA-pure (GE healthcare, USA) equipped with Source30-RPC (GE healthcare, USA) reversed-phase chromatographic column (65mm*10mm Sepax) for fractionation. That is, 0-10% linner is used for gradient elution, and then 10mM Na2HPO4 (pH7.0) buffer containing 20%, 30%, 50% and 60% acetonitrile is used for constant gradient elution, and the flow rate at room temperature is 3ml / min. Finally, the absorbance at 214 and 280 nm was measured. Peak fractions were collected and lyophilized. The lyophilized solution was dissolved in ultrapure water for analysis. For analysis results, see image 3 a.

[0115] (2) Detect the protective effects of the four components A11, A12, A13 and A14 on hydrogen peroxide d...

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Abstract

The present application relates to polypeptides, compositions and applications thereof. The polypeptide is as shown in (i) or (ii): (i) the amino acid sequence is as shown in SEQ ID NO: 1; (ii) the amino acid sequence as shown in SEQ ID NO: 1 is passed through one or several amino acids A polypeptide having the same function as the substitution and / or deletion and / or addition of residues. The polypeptide has the effect of resisting cell death.

Description

technical field [0001] This application generally relates to the field of biopharmaceuticals, and specifically relates to the development and application of small molecular polypeptides, including the dosage form, dosage, and therapeutic effect of the polypeptides. Background technique [0002] Polypeptides are a class of compounds formed by connecting multiple amino acids through peptide bonds, usually consisting of 10-100 amino acid molecules, connected in the same way as proteins, and with a relative molecular mass of less than 10,000. In recent years, with the development and maturity of peptide synthesis technology, peptide drugs have become one of the hotspots in drug research and development. Due to their high safety, wide indications, and remarkable curative effect, they have been widely used in tumors, cardiovascular and cerebrovascular diseases, hepatitis, Prevention, diagnosis and treatment of diseases such as diabetes and AIDS. And these polypeptides play a key ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78A61K47/64A61K49/00A61P3/10A61P7/00A61P9/10A61P25/14A61P25/16A61P25/28A61P31/04A61P35/00A61P37/02
CPCC07K14/78A61K47/6435A61K49/0056A61P31/04A61P7/00A61P9/10A61P25/28A61P25/16A61P25/14A61P37/02A61P35/00A61P3/10
Inventor 韩晶杨泽刘磊
Owner 北京鲲达宇科技有限公司
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