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A three-dimensional hydrogel-graphene-based biosensor and its preparation method
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A biosensor, graphene-based technology, applied in the field of biosensors, can solve problems affecting sensor stability and sensitivity, sensor failure, etc.
Active Publication Date: 2022-02-18
HARBIN INST OF TECH
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During the whole process of detection using this surface sensor, the graphene surface is always exposed to the solution to be tested, while the actual biological solution samples, such as blood, sweat and saliva, contain many non-target biological macromolecules or impurities, which are easy to detect. Form biofouling on the surface of graphene, affect the stability and sensitivity of the sensor, and even cause the sensor to fail
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[0038] The present invention provides a method for preparing a three-dimensional hydrogel-graphene-based biosensor described in the above technical solution, comprising the following steps:
[0039] Prepare an electrode layer on one side of the substrate to obtain a substrate-electrode layer device;
[0040] Arranging a graphene film on the surface of the electrode layer in the base-electrode layer device to obtain a base-electrode layer-graphene film device;
[0041] The reaction solution is subjected to in-situ polymerization on the surface of the graphene film in the substrate-electrode layer-graphene film device, and a three-dimensional hydrogel material layer is formed on the surface of the graphene film to obtain a three-dimensional hydrogel-graphene film. Alkenyl biosensor; the reaction solution contains acrylamide monomer, probe molecule modified with acrylamide group, initiator, sodium nitrate and solvent.
[0042] In the present invention, unless otherwise specified...
Embodiment 1
[0054] figure 1 It is a structural schematic diagram of a three-dimensional hydrogel-graphene-based biosensor in this embodiment. In this embodiment, a three-dimensional hydrogel-graphene-based biosensor includes a substrate, an electrode layer, a graphene film, and a three-dimensional water Gel material layer; the substrate is coated with SiO 2 layer silicon wafer, the total thickness of the substrate is 500µm, the SiO 2 The thickness of the layer is 285nm; the electrode layer includes two electrodes arranged in parallel, the electrode gold / chromium composite conductive electrode, the gold / chromium composite conductive electrode includes a chromium layer and a gold layer that are stacked, and the chromium The thickness of the layer is 2nm, and the thickness of the gold layer is 43nm. Specifically, the chromium layer of the gold / chromium composite conductive electrode is contacted with the substrate; the graphene film is formed by single-layer graphene; the three-dimensional ...
Embodiment 2～9
[0061] Three-dimensional hydrogel-graphene-based biosensors were prepared with reference to the method of Example 1, the difference being that the thicknesses of the three-dimensional hydrogel material layers were 0.63mm, 0.78mm, 1.05mm, 1.27mm, 1.6mm, 1.73mm, 1.96mm and 2.35mm.
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Abstract
The invention provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The three-dimensional hydrogel-graphene-based biosensor provided by the present invention includes a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are sequentially stacked; the three-dimensional hydrogel material layer is composed of a three-dimensional network A structured hydrogel material is formed, and the hydrogel material is obtained through polymerization reaction of raw materials including acrylamide monomers and modified probe molecules, and the modified probe molecules are probe molecules modified with acrylamide groups. The three-dimensional hydrogel-graphene-based biosensor provided by the invention has good stability and high sensitivity.
Description
technical field [0001] The invention relates to the technical field of biosensors, in particular to a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof. Background technique [0002] Graphene-based electrical sensors have the advantages of fast response and high sensitivity. They can be used with probe molecules such as nucleic acid aptamers, antibodies, and enzymes that can recognize target substances with high specificity to form graphene-based nanobiosensors. It has important application value in the detection field. [0003] The graphene-based nanobiosensors reported in the existing studies are all surface-affinity sensors (DOI: 10.1021 / acsami.7b07684), that is, the probe molecules are anchored on the graphene surface through linking molecules containing aromatic ring structures, and the probe molecules are anchored on the surface of graphene. Identify the captured marker molecules with weak charges in the solution to be tested. Under ...
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