Parameters for concentration and washing of particles with acoustics
A technology for washing particles and particles, applied in chemical instruments and methods, biochemical cleaning devices, cleaning methods using liquids, etc., can solve problems such as cost and timeliness that are not conducive to rapid or low-cost preparation of cells, exposure, etc.
Pending Publication Date: 2021-10-19
FLODESIGN SONICS
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Problems solved by technology
Also, the filtration process can be quite ineffective and may require a non-sterile invasive environment for batch processing whereby the cell culture is exposed to external cellular influences or pathogens that may be detrimental to the target cell culture
Additionally, during these physical filtrations, biowaste is generated due to the use of multiple physical filters, which can result in additional steps for proper disposal
The cost and timeliness of this process also hinders the process of quickly or inexpensively preparing cells for introduction into patients
Method used
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[0097] The acoustophoresis system of the present disclosure was tested for its ability to concentrate Jurkat T cells. Jurkat T cells have a diameter of 11 micrometers (μm) to 14 μm. Cell density reduction was measured using an acoustophoresis device and using a Beckman Coulter Vi-CELL Z under various test conditions.
[0098] In a first test A, T cells were concentrated and the cell density of the infiltrate was measured. Dashed lines indicate feed cell density. Desirably, the cell density in the permeate is as low as possible, indicating that the cells are retained in the concentrate. Figure 18 The curve in shows the results of Experiment A over time. Results showed a very low cell density in the permeate, ranging from 0-200,000 cells / mL, indicating that the majority of cells were in the concentrate. The results also showed a high percent permeate reduction, ranging from 80% to 99%.
[0099] In a second experiment B, T cells were concentrated and the cell density of the...
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Abstract
Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.
Description
Background technique [0001] Concentrating therapeutic cells and transferring them from one solution to another (commonly called washing) are two processes that involve multiple stages in the production and use of cells. Washing and separation of materials in cell processing is an important part of the overall efficacy of the cell therapy of choice. In particular, therapeutic cells may be initially suspended in growth serum or in a preservative material such as dimethyl sulfoxide (DMSO). Separating the cells from these fluids enables the cells to be further processed and is important throughout the therapeutic process using such cellular material. In one example, cells are typically recovered from the bioreactor, concentrated, and transferred from culture medium to electroporation buffer prior to transduction (eg, in making CAR-T cells). After cell expansion in the final manufacturing step, they are concentrated and transferred into appropriate solvents depending on the desir...
Claims
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IPC IPC(8): C12N5/00C12M1/42C12M1/00C12M1/36
CPCC12M47/02A61M1/3678B08B3/12C12M41/48C12N5/0068C12M35/04C12M29/18C12M23/02C12N2521/10C12N2509/10B01D21/283C12N13/00
Inventor B·罗斯-约翰斯鲁德B·利普肯斯
Owner FLODESIGN SONICS