Brain glioma biomarker and application thereof

A biomarker, glioma technology, applied in the field of lncRNA-encoded polypeptide biomarkers, which can solve the problems of difficult brain tumors and lag in liquid biopsy

Pending Publication Date: 2021-10-22
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the blood-brain barrier between brain tissue and blood, it is difficult for key information of brain tumors to enter the blood

Method used

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  • Brain glioma biomarker and application thereof
  • Brain glioma biomarker and application thereof
  • Brain glioma biomarker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] Such as figure 1 As shown in A, the construction of non-coding RNA-encoded polypeptide database: first download all human lncRNA transcripts from the NONCODE database (NONCODEV4), and then use ORFfinder (v0.3.0) to scan all lncRNA transcripts to obtain Kozak sequences and the open reading frame (ORF) initiated by AUG. For non-AUG starting sequences, use the six-frame translation mode to perform frameshift reading frames on all transcripts, and use the stop codon as the segmentation site of the non-AUG starting ORF, and extract the space between the two stop codons The region is used as a potential ORF, and then all potential lncRNAs ORFs obtained by ORFfinder and six-frame translation mode are combined, and the potential lncRNA-encoded polypeptide data set is obtained by theoretical translation, and polypeptides with less than 6 amino acids and more than 200 amino acids are further excluded. After removing redundancy, the theoretical lncRNA-encoded polypeptide database...

Embodiment 2

[0033] Analysis and identification of plasma extracellular vesicle lncRNA-encoded polypeptides from glioma patients and healthy controls figure 1 As shown in B, the plasma / serum extracellular vesicles obtained in vitro from the target patient were further analyzed and identified by LC-MS / MS for lncRNA-encoded polypeptides, and healthy samples were used as controls. The specific experimental steps are as follows:

[0034] 1) Take 100 μL of plasma from each patient sample or healthy sample, perform ultracentrifugation at 2500×g for 10 minutes to remove dead cells and platelets, transfer the supernatant to a new EP tube, and add an equal volume of PBS solution filtered through a 0.22 μm filter membrane Make a dilution.

[0035]2) Using asymmetric flow field combined with ultraviolet absorption detector and multi-angle excitation light scattering detector (AF4-UV / MALS) to separate and enrich extracellular vesicles in plasma samples. The conditions are as follows: a polyester film...

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Abstract

The invention relates to the technical field of biological medicines, in particular to a long non-coding RNA (long non-coding RNA, lncRNA) coding polypeptide biomarker for brain glioma diagnosis and application thereof The invention relates to a biomarker of brain glioma. The biomarker is selected from one or more of lncRNA coding polypeptides in the following table; the biomarker is used for early diagnosis and/or recurrence detection of brain glioma. Based on the phenomenon that part of lncRNA coding polypeptide specifically exists in plasma/serum extracellular vesicles obtained in vitro by a glioma patient, and the phenomenon that specificity does not exist in plasma/serum extracellular vesicles obtained in vitro by normal people and other tumor patients, so that the lncRNA coding polypeptide can be used as a tumor marker of the glioma, the occurrence of the brain glioma can be quickly, accurately and clearly determined by detecting the expression profile and the expression quantity of the lncRNA coding polypeptide in plasma/serum extracellular vesicles obtained in vitro by a target patient through mass spectrometry.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an lncRNA-encoded polypeptide biomarker for the diagnosis of glioma and its application. Background technique [0002] Glioma is the most common primary intracranial malignant tumor clinically. The incidence of glioma is 5-8 per 100,000 population, and the 5-year mortality rate is second only to pancreatic cancer and lung cancer among systemic tumors, ranking third. Affected by various physical, chemical, genetic and other factors, the incidence of glioma often ranks first in intracranial tumors, accounting for about 80% of brain tumors. Glioma has the characteristics of insidious onset, fast growth, easy invasion and metastasis, high fatality rate, and short average survival period. It has become a type of disease that causes great harm to human life and health. Early detection and diagnosis of glioma Glioma is the core link to improve the prognosis of glioma patients. ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57407G01N33/57488G01N33/68G01N2333/47
Inventor 杨福全蔡潭溪张宏伟宁威海陈润生骆健俊王继峰郭晓静张青吴博雯李娜张婷婷
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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