HCV (hepatitis C virus) cracking agent, preparation method thereof and HCV detection kit

Abstract

The invention relates to the technical field of biological detection and particularly relates to an HCV (hepatitis C virus) cracking agent, a preparation method thereof and an HCV detection kit. The HCV virus cracking agent comprises an anionic surfactant, a nonionic surfactant, a protein settling agent, protective protein and a cleaning antibody, wherein the nonionic surfactant is N, N-dimethyl-N-(3-sulfopropyl)-1-octadecane ammonium inner salt. The HCV virus splitting agent can effectively split the virus and dissociate an antigen-antibody complex, so sensitivity of detecting the core antigen of the HCV virus is obviously improved, and the probability of illness state delay caused by leak detection in a window period is greatly reduced; the method has advantages of simplicity and rapidness in operation, low cost, high applicability and easiness in popularization and application.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to an HCV virus lysing agent, a preparation method thereof, and a HCV virus detection kit. Background technique [0002] Hepatitis C virus, referred to as hepatitis C, is caused by hepatitis C virus (HCV) infection. Most of the infected people will turn to chronic infection, and nearly half of them will develop into chronic hepatitis. Some of them will eventually develop into liver cirrhosis or even Hepatocellular carcinoma (HCC) has a high incidence and mortality. The detection of HCV is of great significance for the early diagnosis of hepatitis C virus infection and the guidance of clinical treatment. However, as a hepatotropic chronic virus, the onset and clinical symptoms of patients with HCV infection are extremely atypical, and subclinical infection is more common. It is easy to cause missed detection. [0003] The methods currently used for HCV detection mainly include:...

AI Technical Summary

Problems solved by technology

At present, there are two main forms of detection markers for HCV core antigen detection kits reported, one is HCV free core antigen detection, this detection method does not need to lyse the sample, but the detection sensitivity is low; the other is HCV total core antigen detection, this method requires pretreatment of the sample, and the detection sensitivity is high, currently adopted by most manufacturers

However, HCV total core antigen often exists in the form of a complex with antibodies, which needs to be lysed for detection to ensure high detection sensitivity. However, there is still a lack of effective cleavage of HCV core antigen-antibody complexes. sample diluent

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