Test strip for semi-quantitatively detecting amantadine and preparation method and application of test strip

A technology for semi-quantitative detection of amantadine, applied in the field of test strips for semi-quantitative detection of amantadine and its preparation, can solve problems such as complex operation procedures, expensive instruments and equipment, and difficulties in meeting large-throughput market monitoring. Achieve the effects of broad application prospects, simple use, and improved detection efficiency

Inactive Publication Date: 2021-10-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, traditional amantadine detection methods include chromatography, enzyme-linked immunosorbent assay, spectroscopy, and electrochemical sensor detection methods. Th

Method used

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  • Test strip for semi-quantitatively detecting amantadine and preparation method and application of test strip
  • Test strip for semi-quantitatively detecting amantadine and preparation method and application of test strip
  • Test strip for semi-quantitatively detecting amantadine and preparation method and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The invention provides a kind of preparation method of the test paper strip of semi-quantitative detection amantadine, it comprises the steps:

[0051] On the bottom plate, the sample pad, colloidal gold binding pad, nitrocellulose membrane and water-absorbing pad are spliced, and the acid cellulose membrane is coated with four detection lines and quality control lines.

[0052] It should be noted that, the above-mentioned bonding can be fixed and bonded with a special fixing tape for test strips, or can be bonded in other ways such as glue strips as required.

[0053] Optionally, place the nitrocellulose membrane stably, wear protective gloves to press the upper part of the colloidal gold binding pad to the lower part of the nitrocellulose membrane and fix it, and press the upper part of the sample pad to the lower part of the colloidal gold binding pad and fix it; It is 0.2cm; use the special fixing tape stickers for test strips to fix the nitrocellulose membrane, col...

Embodiment 1

[0094] This embodiment provides a test strip for semi-quantitative detection of amantadine, and its structural diagram refers to figure 1 As shown, the T line is the detection line, and the C line is the quality control line.

[0095] The preparation method is as follows:

[0096] (1) Preparation of a conjugate of amantadine antibody and colloidal gold (i.e. gold-labeled antibody conjugate):

[0097] Antibody preparation: The amantadine antibody to be used was dialyzed once with PB solution, then dialyzed twice with distilled water, and the dialysate was changed every 4 hours. After lyophilization, it was dissolved in ultrapure water to 2 mg / mL.

[0098] Screening for optimal labeling conditions: Prepare five 1.5mL centrifuge tubes, add 1mL of colloidal gold to each tube, and 50μL of 0.1M potassium borate solution (use potassium borate solution to adjust the pH of the colloidal gold solution). Gently invert to fully mix the solution to prevent violent shaking from destroyin...

experiment example 1

[0115] In this experimental example, the semi-quantitative gradient amantadine rapid immunoassay test strip prepared in Example 1 was evaluated for specificity and different concentrations of amantadine.

[0116] In this experiment, three other common veterinary drugs: enrofloxacin, ciprofloxacin and ofloxacin were used to test the cross-reactivity of semi-quantitative gradient amantadine test strips.

[0117] The standard samples of enrofloxacin, ciprofloxacin and ofloxacin were diluted to 100ng / mL with PB respectively. Drop 50 μ L of standard sample solution in the sample pad of the test strip prepared by embodiment 1, observe the test strip color within 15 minutes, judge that the test strip has no cross-reaction to other three kinds of veterinary drugs according to the T line without color development (refer to image 3 shown).

[0118] Determination of the semi-quantitative detection range of test strips:

[0119] The semi-quantitative detection range refers to the conce...

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Abstract

The invention discloses a test strip for semi-quantitatively detecting amantadine and a preparation method and application of the test strip, and relates to the field of food safety detection. The test strip can be used for simply, quickly and semi-quantitatively detecting amantadine without professional instruments and equipment and professional laboratory auxiliary detection, the use mode is simple and does not need technical training, the result can be directly identified by naked eyes during manual operation and use, and the on-site detection efficiency of amantadine is greatly improved. The result can be conveniently obtained through on-site sampling investigation during market supervision, and detection and result interpretation of a to-be-detected object can be rapidly and on-site realized through the test strip. Meanwhile, the residual content of amantadine can be rapidly and semi-quantitatively judged through four detection lines with different concentration gradients, and the test strip has a wide application prospect in the aspect of rapid immunodetection of amantadine drugs and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a test strip for semi-quantitative detection of amantadine, a preparation method and application thereof. Background technique [0002] Amantadine, also known as amantadine and tricyclodecanylamine, can promote the operation of dopaminergic nerve endings in the striatum, and is beneficial to the interaction between dopamine and catecholamines in the central nervous system, thereby increasing the content of dopamine in neurons; in addition, Amantadine can also be used as an antiviral drug to close the channel on the host cell membrane, block the entry of RNA viruses into the host cell, and interfere with the replication process of the virus. Due to the occurrence of the A2 influenza pandemic in many countries in the 1960s and 1970s, the U.S. FDA announced that amantadine was widely used as an antiviral drug for the prevention and treatment of influenza. H1N1 virus-induced bir...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558G01N33/543G01N33/52
CPCG01N33/94G01N33/558G01N33/587G01N33/54346G01N33/52
Inventor 邱静宁香雪贾琪钱永忠
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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