A kind of nucleus-targeted carbon dots, preparation and application
A technology of cell nuclei and carbon dots, applied in nano-carbon, fluorescence/phosphorescence, instruments, etc., can solve the problems of low fluorescence quantum yield, insufficient imaging, and long time of entry into cells, and achieve high chemical stability and photostability Good, fast cell entry time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] The preparation method of carbon dots, the specific steps are as follows:
[0042] Semicarbazide hydrochloride (0.0270 g) and m-phenylenediamine (0.0170 g) were dissolved in absolute ethanol (3 ml) to prepare a precursor mixture. After sonication for five minutes, the mixture was transferred to a Teflon-lined in the autoclave. Subsequently, the autoclave was placed in an oven and heated under reflux at 180° C. for 12 hours. After the reaction kettle was naturally cooled to room temperature, a brown solution was obtained, and then the obtained solution was purified by silica gel column chromatography using dichloromethane and methanol as eluents. The organic solvent in the obtained carbon dot solution was removed with a rotary evaporator to obtain a carbon dot solid. The carbon dot solids were dissolved in DMSO to obtain a carbon dot stock solution at a concentration of 2 mg / mL.
Embodiment 2
[0044] To study the stability of carbon dots to common metal ions and amino acids in cells, the specific steps are as follows:
[0045] A certain amount of the carbon dot stock solution was added to the PBS buffer solution to make the concentration 10 μg / mL, and a certain amount of metal ions and amino acids were added to make the concentrations of metal ions and amino acids 100 μM and 1 mM, respectively. The resulting solution was transferred to a fluorescence cuvette, and the fluorescence intensity was measured with a fluorescence spectrophotometer. like figure 1 As shown, after adding metal ions or amino acids, the fluorescence intensity of Cdots did not change significantly, indicating that Cdots have good stability to common metal ions and amino acids in cells.
[0046] The stability of carbon dots on common ROS during cell ferroptosis is studied. The specific steps are as follows:
[0047]A certain amount of carbon dots stock solution was added to PBS buffer solution t...
Embodiment 3
[0049] The rapid cell imaging study of carbon dots, the specific steps are as follows:
[0050] HepG2 cells were seeded in confocal dishes at 37 °C, 5% CO. 2 , cultured in a humidified cell incubator for 24 h (the cell culture medium consists of DMEM high-glucose medium, 10% gibco serum, 100 μg / mL penicillin and 100 μg / mL streptomycin); It was added to a confocal culture dish to make the final concentration 4 μg / mL, excited with a light source with an excitation wavelength of 458 nm, and confocal imaging was performed to observe the time when the carbon dots entered the cells. like image 3 As shown, after co-incubating the carbon dots with cells for 5 min, the whole nucleus has shown green fluorescence, which indicates that the carbon dots have easily penetrated the cell membrane and entered the nucleus. A plateau is reached in about minutes, which indicates the ability of carbon dots to rapidly image nuclei.
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More