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Immunochromatographic analysis method and test strip for use in said method

A technology of immunochromatography and assay method, applied in the field of test strips, can solve problems such as hindering accurate diagnosis and non-specific reactions, and achieve the effect of inhibiting non-specific reactions

Inactive Publication Date: 2021-11-05
SEKISUI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such immunoassays are often accompanied by nonspecific reactions in cases where samples contain contaminants such as cross-antigenic bacteria, viruses, and proteins
This is problematic because such false-positive reactions may prevent accurate diagnosis

Method used

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  • Immunochromatographic analysis method and test strip for use in said method
  • Immunochromatographic analysis method and test strip for use in said method
  • Immunochromatographic analysis method and test strip for use in said method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] [Test Strip Preparation Example 1] Preparation of Immunochromatography Test Strip

[0102] 1) Preparation of colloidal gold particle-labeled anti-influenza virus monoclonal antibody (conjugate)

[0103] Prepare PBS containing anti-influenza A virus monoclonal antibody at a concentration of 100 µg / mL and PBS containing anti-influenza B virus monoclonal antibody at a concentration of 100 µg / mL. To 20 mL of 1OD / mL colloidal gold particle solution was added 1 mL of each antibody solution, and the resulting mixture was stirred at room temperature for 10 minutes. Add 2 mL of 10% bovine serum albumin (BSA) solution to each of the colloidal gold particle / anti-influenza virus monoclonal antibody mixture and the gold colloid particle / anti-influenza virus monoclonal antibody mixture, and mix each obtained mixture Stir for 5 minutes, then centrifuge at 10,000 rpm for 45 minutes at 10°C to obtain a precipitate (conjugate). To each obtained conjugate was added a conjugate dilution ...

Embodiment 2

[0128] 1. Test method

[0129] The detection tests of influenza A virus and influenza B virus were carried out in the same manner as in Example 1 except that different types and concentrations of surfactants were used.

[0130] 2. Test results

[0131] The results of judging whether there is color development in the test line B indicating the detection of influenza B virus are shown in Table 4 and Table 5.

[0132] [Table 4]

[0133]

[0134] [table 5]

[0135]

[0136] In Table 4 and Table 5, "-" indicates that test line B did not develop color. Test line B indicates detection of influenza B virus. Therefore, "-" indicates the absence of false positive results. "+" indicates line coloration, indicating the occurrence of false positive results. None of the samples tested showed a non-specific response to influenza B virus.

[0137] Therefore, it was found that anionic surfactants are effective for suppressing nonspecific reactions occurring in the detection of inf...

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Abstract

The present invention addresses the problem of providing an immunochromatographic analysis method and a test strip for use in the method such that non-specific reactions are suppressed. The problem can be solved by an immunochromatographic analysis method for a substance to be detected using a test strip comprising the following (1) to (3), (1) A sample pad having a sample supply part for supplying a biological sample that may contain the substance to be detected. (2) An insoluble membrane carrier having at least one detection part at which a binding partner immunologically reactive with the substance to be detected is immobilized. (3) A conjugate in which the binding partner immunologically reactive with the substance to be detected is immobilized to a label body, the method being characterized in that a conjugate is retained together with an anionic surfactant at a conjugate immobilization part.

Description

technical field [0001] The present invention relates to an immunochromatographic assay method for detecting an analyte, such as influenza virus, and a test strip for use in the method. The invention also relates to methods of inhibiting non-specific reactions in immunochromatographic assays for the detection of analytes such as influenza virus. Background technique [0002] For the purpose of early diagnosis of disease, POCT (point-of-care testing) is performed in real time at the patient's location to quickly obtain useful information for diagnosis and treatment. In POCT, immunoassays for the detection of disease-causing analytes are widely performed using binding partners that specifically react with the analyte. Among these immunoassays, immunochromatography is particularly widely used because of its simple operation. However, such immunoassays are often accompanied by nonspecific reactions in cases where samples contain contaminants such as cross-antigenic bacteria, vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/569
CPCG01N33/54387G01N33/5306G01N33/56983G01N2333/08G01N33/54388
Inventor 东加奈子家治翔平森田元喜
Owner SEKISUI MEDICAL CO LTD