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Preparation method and application of microneedle loaded with nano-motor driven exosome for treating enthesiopathy

A motor-driven, exosome technology, applied in the directions of microneedles, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., to achieve good therapeutic effect, improve therapeutic effect, and avoid the effect of being easy to fall off

Pending Publication Date: 2021-11-12
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are a lot of reactive oxygen species (ROS) and nitric oxide synthase (NOS) in the terminal disease microenvironment, so nitric oxide (NO) gas can be produced by reacting L-arginine+ROS / NOS→NO↑+L-citrulline, And NO gas is used as the driving force, but L-arginine cannot be directly combined with EXO

Method used

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  • Preparation method and application of microneedle loaded with nano-motor driven exosome for treating enthesiopathy
  • Preparation method and application of microneedle loaded with nano-motor driven exosome for treating enthesiopathy
  • Preparation method and application of microneedle loaded with nano-motor driven exosome for treating enthesiopathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This embodiment provides a method for preparing a nanomotor-driven exosome (EXO / MBA) microneedle specially used for the treatment of terminal diseases, which specifically includes the following steps:

[0044] Step 1, extraction of tendon stem cell exosomes (EXO).

[0045] SD rats aged 6-8 weeks were selected, and after adapting to the environment, they were killed by excessive injection of chloral hydrate with a mass volume concentration of 10% under aseptic conditions, and the heels of SD rats were cleaned with a volume concentration of 75% and cut open. The skin on the surface of the tendon, the fascia and muscle were peeled off, the Achilles tendon of SD rats was taken out, washed with PBS solution and placed on gauze, and the Achilles tendon was transferred to a large dish in an ultra-clean bench and shredded; a sufficient amount of 3mg / mL Type I collagenase solution, select a filter membrane with a pore size of 0.22 μm to sterilize the type I collagenase solution,...

Embodiment 2

[0056] The microneedles loaded with EXO / MBA prepared in Example 1 were applied to establish a rat model for treating Achilles tendinopathy, such as Figure 8 shown.

[0057] SD rats at 24-48 weeks were selected for this experiment, and Achilles tendons with intact Achilles tendon and Achilles tendinopathy were used as control groups. A sufficient amount of 5 mg / ml type I collagenase solution was prepared, and a filter with a pore size of 0.22 μm was used. Sterilize by membrane filtration, inject locally on one side of the Achilles tendon of both feet of the rat for 2 weeks, inject once every other day, and the single side dose is 60 μl to obtain the Achilles tendinopathy model of the rat; the volume concentration is 75% The alcohol solution disinfected the skin of the Achilles tendon of rats, and the microneedles loaded with EXO / MBA prepared in Example 1 were attached to the affected parts of the rats, pressed properly and tied with adhesive tape, and the bottom of the micron...

Embodiment 3

[0079] This embodiment provides a method for preparing a nanomotor-driven exosome (EXO / MBA) microneedle specially used for the treatment of terminal diseases, which specifically includes the following steps:

[0080] Step 1, extraction of tendon stem cell exosomes (EXO).

[0081] SD rats aged 6-8 weeks were selected, and after adapting to the environment, they were killed by excessive injection of chloral hydrate with a mass volume concentration of 10% under aseptic conditions, and the heels of SD rats were cleaned with a volume concentration of 75% and cut open. The skin on the tendon surface, the fascia and muscle were peeled off, the Achilles tendon of SD rats was taken out, rinsed with PBS solution and placed on gauze, the Achilles tendon was transferred to a large dish in an ultra-clean bench and shredded; a sufficient amount of 5mg / mL Type I collagenase solution, select a filter membrane with a pore size of 0.22 μm to sterilize the type I collagenase solution, add it to ...

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Abstract

The invention provides a preparation method and application of microneedle loaded with nano-motor driven exosome for treating enthesiopathy, which comprises the following steps: firstly, extracting a tendon stem cell exosome from achilles tendon, then in-situ polymerizing and wrapping the exosome through 2-methacryloyloxyethyl phosphorylcholine, and grafting L-arginine to obtain the nano-motor driven exosome; and mixing the nano-motor driven exosome with methylacrylic acid acylated gelatin and polyvinyl alcohol, adding the mixture into a microneedle template, finally preparing the microneedle loaded with the nano-motor driven exosome, and the microneedle is applied to preparation of a medicine for treating enthesiopathy. The microneedle loaded with the nano-motor driven exosome take nitric oxide gas generated by reaction as driving force of the exosome, the exosome retained in the epidermal layer in the microneedle is delivered to the damaged tendon part by combining the property that the nano-motor attaches to the damaged part with the reduced pH value, and by combining the anti-inflammatory effect of the nitric oxide gas, the treatment effect of the enthesiopathy is effectively improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method and application of a nanomotor-driven exosome microneedle specially used for treating terminal diseases. Background technique [0002] Enthesiopathy is one of the common sports system diseases, such as supraspinatus tendinitis, tennis elbow, Achilles tendinopathy, etc. all fall into this category. Conventional treatment methods include oral anti-inflammatory drugs, local plasters, rest, and physical therapy, which have obvious gastrointestinal side effects, insufficient drug penetration, and poor compliance. to formulate a treatment plan. [0003] Exosomes (Exosomes, EXO) have obvious anti-inflammatory and damage-repairing effects, but local injection of EXO to treat terminal diseases will cause pain, cause secondary damage, and require repeated injections. Microneedle is a painless and controllable transdermal drug delivery method. Delivery ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K35/32A61K33/00A61K47/32A61K47/42A61P29/00A61P19/04A61M37/00
CPCA61K9/0021A61K35/32A61K33/00A61K47/32A61K47/42A61P29/00A61P19/04A61M37/0015A61M2037/0023A61M2037/0046A61M2037/0053A61M2037/0061
Inventor 史冬泉刘岸龙万密密
Owner NANJING DRUM TOWER HOSPITAL
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