3 results about "Calcanean tendon" patented technology

This invention relates to the field of medical materials technology, and more particularly to an inner and outer layer filling material for a composite artificial tissue microparticle scaffold and its preparation method. The method includes: first, modifying bovine Achilles tendon type I collagen and chondroitin sulfate with thiolation, then mixing and reacting them with corresponding components to granulate, thus obtaining an inner layer of injectable microparticle gel; then preparing boric acid-modified ε-polylysine, separately preparing a dense moisturizing gel membrane and a gradient porous drainage sponge, bonding the two together at the interface, and drying to obtain the final product. This invention, through the dual cross-linking structure design of the inner layer of injectable microparticle gel and the synergistic construction with the outer double-layer membrane, significantly improves the mechanical strength, degradation rate, matching with new tissue growth, and biocompatibility of the composite material.

The present application belongs to the field of material chemistry and biology technology, and particularly relates to a composite film and a preparation method and application thereof. The HOFilms material of the present application uses a mild and simple preparation method to prepare a high-quality, uniformly distributed hydrogen-bonded organic framework film, effectively retaining the biological function of the material; the production process is simple, raw materials are easy to obtain, and the material can be produced in large scale in a factory; the material can be used as a physical barrier or a support to regulate the repair microenvironment at the molecular level, and further form an integrated active Achilles tendon regeneration platform from mechanical support to biological treatment; the HOFilms material has a "wet response" intelligent property: not only can it stably exist in a wet environment in vivo, but also can utilize or respond to moisture in the environment (such as swelling, shrinking, releasing factors) to perform specific functions, thereby interacting with dynamic physiological processes; the HOFilms material itself has an active biological function, that is, improving the inflammatory microenvironment and directly promoting the regeneration of tendon cells, thereby fundamentally overcoming the healing barrier.

The application discloses a medical collagen extraction method and belongs to the technical field of biomedical materials. The extraction steps are as follows: after removing surface foreign matters from a bovine Achilles tendon, the bovine Achilles tendon is cut into particles, washed by a NaCl solution and soaked by a Tris-HCl buffer solution containing EDTA-Na2; the pretreated bovine Achilles tendon tissue is added into a swelling treatment liquid prepared by taking lactic acid and betaine as raw materials to perform soaking, so that swelled bovine Achilles tendon tissue is obtained; the swelled bovine Achilles tendon tissue is subjected to enzymolysis by pepsin, so that a crude collagen extraction liquid is obtained; poly(N-isopropyl acrylamide) is added into the crude collagen extraction liquid to precipitate a collagen-containing precipitate; after resuspension of the precipitate, constant-volume ultrafiltration diafiltration purification is performed, so that a collagen concentrate liquid is obtained, and the medical collagen extraction is completed. The product has the characteristics of high purity, structural integrity and low endotoxin, meets medical-grade safety standards and is suitable for large-scale popularization and use.