Hypsizygus marmoreus strain

The technology of mushroom and strain is applied in the field of edible fungus, which can solve the problems of mushroom caps with nodules, poor quality, low yield per unit, etc., and achieve the effects of good quality, long shelf life and delicious taste.

Pending Publication Date: 2021-11-12
西充星河生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the strains used by domestic Shiji mushroom manufacturers are basically from Japan. The dominant strains of Shiji mushrooms currently in use are numbered wz-29, wz-65, wz-77; the fruiting performance of strain wz-29 Because of good quality, the yield per unit area is relatively low; wz-65 and wz-77 have relatively high yield per unit area, but the mushroom cap has a wart cap and a short shelf life, and the quality is relatively poor; Shimeji mushroom strain

Method used

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  • Hypsizygus marmoreus strain
  • Hypsizygus marmoreus strain
  • Hypsizygus marmoreus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Monospore hybridization

[0027] The present invention selects six kinds of domestic mushroom varieties (wz-29, wz-65, wz-77, WB-19, WB-86, XCHW-1, the above mushroom strains can be purchased through commercial channels) to carry out The spores were collected, single spores were picked by dilution coating, and the single spores were determined to be single spores by microscopic observation without lock-like association. Using single spore wz-29 as the male parent, single spore wz-65, wz-77, WB-19, WB-86, XCHW-1 as the female parent for cross breeding, and finally through the fruiting of each heterozygous successful breeding As a result, a new strain of jiji mushroom with no tumor cap and high yield was selected from the hybridization of wz-29 and WB-86, and the number was jiji mushroom XCXH-2.

[0028] (2) Study on the characteristics of Shiji mushroom XCXH-2

[0029] ISSR and RAPD identification

[0030] The experimental medium preparation method is as follows: ...

Embodiment 2

[0044] (1) Mycelia growth

[0045] Within the sterile area formed by the alcohol lamp, scrape off the weak mycelium and mycelium lying on the wall at the front end of the test tube with an inoculation needle after burning and cooling, and cut the upper part of the front end and the rear end into 3*3mm Small pieces of uniform size are placed on a plate with a distance of 4cm between the two bacterial blocks. After the inoculation is completed, mark the batch on the transferred plate, wrap it in a fresh-keeping bag and put it in a basket, cover it with newspaper, and put it in a biochemical culture medium. Cultivation in an oven at a temperature of 21°C. During the cultivation process, measure and record the diameter of the colony every day, observe the shape, color and growth of the mycelium; when the mycelium is full of the plate, the culture is terminated. Calculate the mycelial growth rate, and the calculation formula is: mycelial growth rate (cm / d)=colony diameter (cm) / gro...

Embodiment 3

[0062] Freshness test

[0063] The XCXH-2 strain harvested 24 flowers on the 23rd day of growth. The packaging workshop cut the roots and packed them in boxes, electronically weighed them, and controlled the boxed weight at 150g-160g. The boxed machines were plastic-packed, and the temperature in the packaging workshop was 14-16°C. Packing time 1 hour.

[0064] The packaged boxed mushrooms are packed in foam boxes, sealed with scotch tape and placed in the sample rack of the cold storage. The temperature of the warehouse is set at 2-4 °C. The color, freshness, smell, and mushroom quality of the mushroom cap and mushroom handle were recorded by unpacking the bag every other week. The results are shown in Table 6.

[0065] The fresh-keeping situation of XCXH-2 of different fresh-keeping days of table 6

[0066]

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Abstract

The invention discloses a hypsizygus marmoreus strain. The hypsizygus marmoreus strain is hypsizygus marmoreus XCXH-2, the preservation number is CCTCC M 2020068, the preservation date is April 17, 2020, the preservation address is Luojia mountain, Wuchang, Wuhan, Hubei, and the preservation unit is China Center for Type Culture Collection. The hypsizygus marmoreus XCXH-2 provided by the invention has the advantages that the spawn running speed is higher than that of a parent strain, the per unit yield is higher than that of the parent strain, the quality is excellent, a mushroom cap is not easy to open, patterns are clear, mushroom stems are white and robust, the shelf life is long, the appearance is attractive, the taste is delicious, concentrated cultivation and repeated cultivation can be realized, the stability is high, the growth is neat, and thus, the hypsizygus marmoreus XCXH-2 can be used as a production seed source of hypsizygus marmoreus factory production enterprises and a seed breeding dominant seed source of research and development units.

Description

technical field [0001] The invention belongs to the technical field of edible fungus strains, and in particular relates to a true mushroom strain. Background technique [0002] Shiji mushroom is a rare edible fungus introduced from Japan, also known as: crab-flavored mushroom, seafood mushroom, Hongxi mushroom, jade mushroom, belonging to Basidiomycotina, Phylomycetes, Agaricaceae, White Mushrooms, Liphyllum family, Yu mushroom genus. According to Yang Zhuliang's report, there are also wild shimeji mushrooms in Yunnan Province, but the shimeji mushrooms cultivated in various places are all from Japan. [0003] In 1972, Nippon Takara Shuzo Co., Ltd. first succeeded in artificially cultivating Shimeji mushroom and obtained a patent right. It has been put into production in Nagano Prefecture since 1973. In the 1980s, my country introduced Shiji mushrooms, mainly in Shanxi, Hebei, Henan, Shandong, and Fujian for small-scale cultivation. In 2001, Shanghai, Guangdong and other p...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12R1/645
CPCA01H15/00
Inventor 黄清华陈长青许喜佳江萍蒋小庆尹军王志学严会李挺丁祥
Owner 西充星河生物科技有限公司
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