Cherry blossom SSR molecular marker primers and application thereof in identification of 20 cherry blossom varieties
A molecular marker and variety identification technology, applied in the biological field, can solve the problems of low resolution of molecular markers, insufficient identification and distinction of all varieties, etc., and achieve the effect of improving identification efficiency and enriching types
Pending Publication Date: 2021-11-23
ZHEJIANG FORESTRY ACAD
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Problems solved by technology
However, due to the variety of cherry blossoms, the existing molecular markers have low resolution and are not enough to identify and distinguish all varieties. Therefore, the continuous development of new molecular markers is very necessary for the identification of cherry blossom varieties
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[0022] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.
[0023] Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art. The test methods in the following examples are conventional methods unless otherwise specified. Unless otherwise specified, the reagents and materials used can be obtained through commercial channels.
[0024] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can also be applied in the present invention. The preferred implementation methods and materials described herein are for demonstration purp...
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The invention discloses cherry blossom SSR molecular marker primers and application thereof in identification of 20 cherry blossom varieties. The cherry blossom SSR-related molecular marker primers are independently developed from the genome data of the prunus caudatus, amplification and polymorphic screening are performed on the 16 pairs of developed SSR primers, and finally, the primers ZJF01 and ZJF02 are selected to identify 24 varieties. The result proves that the 20 cherry blossom varieties can be distinguished and identified by using the two pairs of primer combinations. Thus, the 20 cherry blossom varieties can be identified by using fewer primer combinations, thereby enriching the types of molecular marker identification of the cherry blossom varieties. The selected primer pairs are fewer, thereby greatly enhancing the identification efficiency.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cherry blossom SSR molecular marker primer and its application in the identification of 20 cherry blossom varieties. Background technique [0002] Cherry blossom is the collective name for the subgenus Cerasus of the genus Prunus sensu lato in the Rosaceae family. Cherry blossom is a world-renowned flower viewing tree. When it is in full bloom, it is full of flowers, and when it is finally in bloom, it is colorful. As a fruit tree with high economic value and elegant garden flowers and trees, it has broad prospects for development and utilization. In the past hundred years, more than 600 cherry garden varieties have been bred through natural variation screening and artificial hybridization. However, due to the high morphological similarity among many varieties, the unknown sources of varieties, and the frequent phenomenon of the same thing with different names and diff...
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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 蒋冬月沈鑫孙泽硕李因刚
Owner ZHEJIANG FORESTRY ACAD