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Strain Phocaeicola sp. capable of relieving ulcerative colitis and application of strain Phocaeicola sp.

A technology of bacterial strains and viable counts, applied in the field of microbiology, can solve problems such as inducing diabetes and affecting the success rate of treatment, and achieve the effects of increasing colon length, relieving lipopolysaccharide infection, and relieving inflammation

Active Publication Date: 2021-11-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have shown that long-term use of traditional mesalamine (5-aminosalicylic acid, hydrocortisone, buninide) and other drugs may cause certain side effects, such as inducing diabetes, hypertension or osteoporosis, affect the success of treatment

Method used

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  • Strain Phocaeicola sp. capable of relieving ulcerative colitis and application of strain Phocaeicola sp.
  • Strain Phocaeicola sp. capable of relieving ulcerative colitis and application of strain Phocaeicola sp.
  • Strain Phocaeicola sp. capable of relieving ulcerative colitis and application of strain Phocaeicola sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Isolation and screening of bacterial strain (Phocaeicola sp.) CCFM1183

[0069] 1. Sample collection

[0070] Stool samples were collected from people in Yining County, Xinjiang Autonomous Region. The samples were placed in stool tubes filled with 30% glycerin and stored in an insulated box with ice packs. After being brought back to the laboratory, they were quickly placed in a -80°C refrigerator for separation and screening.

[0071] 2. Isolation and purification of bacterial strains

[0072] (1) Dilution coating: take about 0.5 g of the content preserved in 30% glycerol and add it into a 10 mL centrifuge tube containing 4.5 mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluent;

[0073] (2) Coating culture: pipette 100 μL of the above 10 -4 、10 -5 、10 -6 The three gradient dilutions were spread evenly on the Bacteroides specific screening medium...

Embodiment 2

[0081] Example 2: Effect of strain (Phocaeicola sp.) CCFM1183 on DSS-induced ulcerative colitis

[0082] Specific steps are as follows:

[0083] 1. Selection and preparation of bacterial strains:

[0084] Streak the strain (Phocaeicola sp.) CCFM1183 on BHIS solid medium, culture at 37°C for 48 hours, and obtain single colonies; pick single colonies and inoculate them in BHIS liquid medium, and culture at 37°C for 18 hours for activation , two generations of continuous activation to obtain activation solution respectively;

[0085] The activated solution obtained was inserted into the BHIS liquid medium with an inoculum size of 2% (v / v), and after anaerobic cultivation at 37°C for 18 hours, the bacterial solution was obtained; the bacterial solution was centrifuged at 6000r / min for 5min to obtain bacterial strains ( Phocaeicola sp.) CCFM1183 cells, wash the cells of the strain (Phocaeicola sp.) CCFM1183 with sterile PBS buffer, collect the sludge, resuspend in PBS buffer cont...

Embodiment 3

[0097] Example 3: Bacterial strain (Phocaeicola sp.) CCFM1183 changes the body weight and colon length of mice with ulcerative colitis induced by DSS

[0098] DSS-induced colitis in mice can lead to weight loss and colon shortening in mice. Therefore, body weight and colon length are important indicators for evaluating the severity of inflammation in colitis mice. After successful modeling, the mice were sacrificed and dissected, and the colon tissue of the mice was taken, and the total length of the colon of each mouse was measured with a ruler.

[0099] weight changes such as figure 1 As shown in Table 2, compared with the normal group, the body weight of the mice in the DSS group decreased significantly, indicating that the ulcerative colitis model was constructed successfully; compared with the DSS group, bacterial strain (Phocaeicola sp.) CCFM1183 significantly increased the body weight of the mice, and its The reduction ratio was 76.64% of the DSS group.

[0100] Table...

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Abstract

The invention discloses a strain Phocaeicola sp. capable of relieving ulcerative colitis and an application of the strain Phocaeicola sp., and belongs to the technical field of microorganisms. The strain (Phocaeicola sp.) CCFM1183 provided by the invention can be used for effectively preventing weight loss and shortening of colon length caused by DSS induction and repairing colonic mucosa injury. Therefore, the strain (Phocaeicola sp.) CCFM1183 capable of relieving the ulcerative colitis, disclosed by the invention, has a wide application prospect in the directions of foods and microecologics.

Description

technical field [0001] The invention relates to a bacterial strain Phocaeicola sp. capable of alleviating ulcerative colitis and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] Ulcerative colitis (UC) is a typical chronic recurrent idiopathic intestinal inflammation, which can cause colonic mucosal inflammation, rectal bleeding, and diarrhea. Ulcerative colitis is often accompanied by intestinal epithelial barrier dysfunction, the main manifestation of which is increased intestinal permeability. Studies have found that compared with normal tissues, the structure and function of tight junctions in ulcerative colitis intestinal tissues are altered, and intestinal mucosal permeability is increased. Given the enormous harm of ulcerative colitis, finding ways to alleviate the severity of ulcerative colitis has attracted widespread attention. In the past, the main method for relieving ulcerative colitis was drug treatment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P1/04A61P1/00A23L33/14A23C9/127A23L11/65A23L2/02A23L2/52A23C17/02A23C9/16A23L19/00C12R1/01
CPCC12N1/20A61K35/741A61P1/04A61P1/00A23L33/14A23C9/127A23L11/65A23L2/02A23L2/52A23C17/02A23C9/16A23L19/00A23V2002/00A23V2400/123A23V2400/249A23V2200/30A23V2250/5118A23V2250/5108A23V2250/1628A23V2250/628Y02A50/30
Inventor 翟齐啸陈卫王晨于雷雷田丰伟陆文伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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